CAJ | 학술논문

目的观察小鼠各组织中锌指蛋白3(zinc-finger BED domain-containing 3, ZBED3)的表达差异及利拉鲁肽、葡萄糖和胰岛素刺激对C57BL/6J和db/db小鼠肝脏ZBED3表达的影响。方法实时定量PCR法比较C57BL/6J小鼠各组织中 ZBED3 mRNA表达差异,Western 印迹检测 ZBED3在 C57BL/6J 和db/db小鼠肝脏的蛋白表达差异及观察不同浓度利拉鲁肽、葡萄糖、胰岛素对C57BL/6J和db/db小鼠肝脏ZBED3表达的影响。结果小鼠肌肉、脾脏、肾脏、脑、脂肪、心脏、肺、肝脏均有ZBED3 mRNA表达,其中肌肉表达最高。与C57BL/6J小鼠相比,db/db小鼠肝脏ZBED3蛋白表达显著升高(P<0.01)。高浓度利拉鲁肽、葡萄糖、胰岛素均能够显著增加db/db小鼠肝脏ZBED3蛋白表达(P<0.05)；其次,高浓度葡萄糖能明显增加C57BL/6J小鼠肝脏ZBED3表达(P<0.01),而高浓度利拉鲁肽、胰岛素对C57BL/6J小鼠肝脏ZEBD3表达无明显影响。结论 ZBED3可能作为一种新因子参与糖代谢,且利拉鲁肽、葡萄糖及胰岛素均能够调节db/db小鼠肝脏ZBED3的表达,因此,本研究推测ZBED3可能在机体糖代谢中发挥着重要的作用。
목적관찰소서각조직중자지단백3(zinc-finger BED domain-containing 3, ZBED3)적표체차이급리랍로태、포도당화이도소자격대C57BL/6J화db/db소서간장ZBED3표체적영향。방법실시정량PCR법비교C57BL/6J소서각조직중 ZBED3 mRNA표체차이,Western 인적검측 ZBED3재 C57BL/6J 화db/db소서간장적단백표체차이급관찰불동농도리랍로태、포도당、이도소대C57BL/6J화db/db소서간장ZBED3표체적영향。결과소서기육、비장、신장、뇌、지방、심장、폐、간장균유ZBED3 mRNA표체,기중기육표체최고。여C57BL/6J소서상비,db/db소서간장ZBED3단백표체현저승고(P<0.01)。고농도리랍로태、포도당、이도소균능구현저증가db/db소서간장ZBED3단백표체(P<0.05)；기차,고농도포도당능명현증가C57BL/6J소서간장ZBED3표체(P<0.01),이고농도리랍로태、이도소대C57BL/6J소서간장ZEBD3표체무명현영향。결론 ZBED3가능작위일충신인자삼여당대사,차리랍로태、포도당급이도소균능구조절db/db소서간장ZBED3적표체,인차,본연구추측ZBED3가능재궤체당대사중발휘착중요적작용。
Objective To compare zinc-finger BED domain-containing 3 ( ZBED3 ) expression in various tissues of C57BL/6J mice and the effects of liraglutide, glucose, and insulin on the levels of ZBED3 protein expression in C57BL/6J mice and db/db mice. Methods The mRNA level of ZBED3 in various tissues of C57BL/6J mice was measured by realtime PCR. The protein level of ZBED3 was measured by using western blot. Results ZBED3 mRNA levels were detected in muscle, spleen, kidney, brain, heart, lung, and liver of C57BL/6J mice, yielding the highest expression in muscle. Additionally, The liver ZBED3 levels were higher in db/db mice compared with C57BL/6J mice (P<0. 01). Furthermore, the protein expression of ZBED3 was significantly increased in liver tissues of db/db mice treated with high concentrations of liraglutide, glucose or insulin(P<0. 05), however, the expression of ZBED3 only responded to high concentration of glucose in liver tissues of C57BL/6J mice. Conclusion ZBED3 may act as a novel factor in regulating glucose metabolism. The expression of ZBED3 can be regulated by liraglutide, glucose, and insulin. Thus, ZBED3 may play an important role in conditioning of hyperglycemia.