中华老年心脑血管病杂志
中華老年心腦血管病雜誌
중화노년심뇌혈관병잡지
CHINESE JOURNAL OF GERIATRIC CARDIOVASCULAR AND CEREBROVASCULAR DISEASES
2014年
8期
863-866
,共4页
高焕民%刘丽丽%齐明山%沈焱
高煥民%劉麗麗%齊明山%瀋焱
고환민%류려려%제명산%침염
大脑中动脉%脑缺血%再灌注%模型 ,动物
大腦中動脈%腦缺血%再灌註%模型 ,動物
대뇌중동맥%뇌결혈%재관주%모형 ,동물
middle cerebral artery%brain ischemia%reperfusion%models,animal
目的：探讨大鼠左右大脑中动脉缺血再灌注模型的差异。方法选择右利雄性Wistar大鼠48只，按照随机数字表分为左侧大脑中动脉缺血再灌注组（左侧优势组）、右侧大脑中动脉缺血再灌注组（右侧非优势组），每组24只，每组使用各自侧别的假手术对照。血管内线栓法阻塞大脑中动脉2h，然后再灌注。测试神经功能，取脑分别进行常规T T C染色和H E染色，测量脑梗死体积，光镜下观察脑组织病理变化。结果左侧优势组再灌注24、48和72 h神经功能缺损评分明显低于右侧非优势组（ P＜0．05）。左侧优势组缺血程度较右侧非优势组重。左侧优势组神经元数量严重缺失，海马细胞排列紊乱，脑梗死体积明显大于右侧非优势组[（102.1±8．8）mm3 vs （97．0±11．2）mm3，P＜0．05]。结论大鼠优势半球大脑中动脉阻塞后，神经功能缺损程度较非优势侧严重，脑梗死体积更大。大鼠优势半球局灶性脑缺血模型重复性好，而且可靠。
目的：探討大鼠左右大腦中動脈缺血再灌註模型的差異。方法選擇右利雄性Wistar大鼠48隻，按照隨機數字錶分為左側大腦中動脈缺血再灌註組（左側優勢組）、右側大腦中動脈缺血再灌註組（右側非優勢組），每組24隻，每組使用各自側彆的假手術對照。血管內線栓法阻塞大腦中動脈2h，然後再灌註。測試神經功能，取腦分彆進行常規T T C染色和H E染色，測量腦梗死體積，光鏡下觀察腦組織病理變化。結果左側優勢組再灌註24、48和72 h神經功能缺損評分明顯低于右側非優勢組（ P＜0．05）。左側優勢組缺血程度較右側非優勢組重。左側優勢組神經元數量嚴重缺失，海馬細胞排列紊亂，腦梗死體積明顯大于右側非優勢組[（102.1±8．8）mm3 vs （97．0±11．2）mm3，P＜0．05]。結論大鼠優勢半毬大腦中動脈阻塞後，神經功能缺損程度較非優勢側嚴重，腦梗死體積更大。大鼠優勢半毬跼竈性腦缺血模型重複性好，而且可靠。
목적：탐토대서좌우대뇌중동맥결혈재관주모형적차이。방법선택우리웅성Wistar대서48지，안조수궤수자표분위좌측대뇌중동맥결혈재관주조（좌측우세조）、우측대뇌중동맥결혈재관주조（우측비우세조），매조24지，매조사용각자측별적가수술대조。혈관내선전법조새대뇌중동맥2h，연후재관주。측시신경공능，취뇌분별진행상규T T C염색화H E염색，측량뇌경사체적，광경하관찰뇌조직병리변화。결과좌측우세조재관주24、48화72 h신경공능결손평분명현저우우측비우세조（ P＜0．05）。좌측우세조결혈정도교우측비우세조중。좌측우세조신경원수량엄중결실，해마세포배렬문란，뇌경사체적명현대우우측비우세조[（102.1±8．8）mm3 vs （97．0±11．2）mm3，P＜0．05]。결론대서우세반구대뇌중동맥조새후，신경공능결손정도교비우세측엄중，뇌경사체적경대。대서우세반구국조성뇌결혈모형중복성호，이차가고。
Objective To compare the rat left and right cerebral middle artery (MCA ) ischemia/reperfusion (I/R) models .Methods Forty-eight male Wistar rats were randomly divided into left MCA I/R group and right MCA I/R group (24 in each group) .The MCA was occluded for 2 h by Longa occlusion and then reperfused .Neurological function of rats was assessed .Rat brain tissue samples were stained with TTC and HE .Ischemic stroke volume was measured and brain lesions were observed under light microscope .Results The neurological scores at 24 ,48 and 72 h were significantly lower in left MCA I/R group than in right MCA I/R group (P<0 .05) .TTC staining demonstrated that the ischemia was severer in left MCA I/R group than in right MCA I/R group (P<0 .05) .HE-stained hippocampus tissue showed that the number of lost neurons was greater , the cells were worse dearranged ,and the ischemic stroke volume was larger (102 .1 ± 8 .8 mm3 vs 97.0 ± 11 .2 mm3 ) in left MCA I/R group than in right MCA I/R group (P<0 .05) .Conclusion T he neurological defect is severer and the ischemic stroke volume is larger in the dominant hemi-sphere than in the non-dominant hemisphere of rats following MCA occlusion .The repeatability of rat focal ischemia model is better and more reliable in dominant hemisphere than in non-dominant hemisphere .
