CAJ | 학술논문

目的：采用反转录聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）检测咬牙合干扰对实验大鼠颞下颌关节软骨的炎性因子表达。方法选用6周龄雌性 SD 大鼠9只，随机分为咬牙合紊乱组、去除咬牙合紊乱组和正常对照组，每组3只。制造咬牙合干扰动物模型。6周后，咬牙合紊乱组不做处理，去除咬牙合紊乱组去除咬牙合干扰，正常对照组不做处理。8周后切取大鼠右侧颞下颌关节，采用 RT-PCR 方法检测颞下颌关节软骨中白细胞介素1（interleukin-1，IL-1），白细胞介素6（interleukin-6，IL-6），肿瘤坏死因子α（tumor necrosis factor α，TNF-α）及γ干扰素（interferon -γ，IFN-γ）的表达。结果在咬牙合干扰条件下，RT-PCR 检测显示炎性因子 IL-1、IL-6及 TNF-α在各组大鼠中均有表达，但咬牙合紊乱组和去除咬牙合紊乱组的炎性因子表达均高于对照组，咬牙合紊乱组 IL-6和 TNF-α的表达高于去除咬牙合紊乱组；3组大鼠均未检测到 IFN-γ的表达。结论咬牙合干扰条件下，炎性因子 IL-1、IL-6及TNF-α在颞下颌关节软骨中的表达增强，提示炎性因子与实验大鼠髁突软骨病理性改建活动有相关性。
목적：채용반전록취합매련반응（reverse transcription polymerase chain reaction，RT-PCR）검측교아합간우대실험대서섭하합관절연골적염성인자표체。방법선용6주령자성 SD 대서9지，수궤분위교아합문란조、거제교아합문란조화정상대조조，매조3지。제조교아합간우동물모형。6주후，교아합문란조불주처리，거제교아합문란조거제교아합간우，정상대조조불주처리。8주후절취대서우측섭하합관절，채용 RT-PCR 방법검측섭하합관절연골중백세포개소1（interleukin-1，IL-1），백세포개소6（interleukin-6，IL-6），종류배사인자α（tumor necrosis factor α，TNF-α）급γ간우소（interferon -γ，IFN-γ）적표체。결과재교아합간우조건하，RT-PCR 검측현시염성인자 IL-1、IL-6급 TNF-α재각조대서중균유표체，단교아합문란조화거제교아합문란조적염성인자표체균고우대조조，교아합문란조 IL-6화 TNF-α적표체고우거제교아합문란조；3조대서균미검측도 IFN-γ적표체。결론교아합간우조건하，염성인자 IL-1、IL-6급TNF-α재섭하합관절연골중적표체증강，제시염성인자여실험대서과돌연골병이성개건활동유상관성。
Objective Reverse transcription polymerase chain reaction(RT-PCR)was used for inflammatory factor expression in experimental rat temporomandibular joint cartilage by the occlusal interference. Methods The six week old female SD 9 rats were randomly divided into occlusal disorder group,removed occlusal disorder group and normal control group,3 rats in each group. The occlusal interference animal model was established. After six weeks,occlusal disorder group did not receive any treatment,removed occlusal disorder group was rid of occlusal interference,normal control group served as normal control. After eight weeks,the rats were sacrificed,the right side of the temporomandibular joint was cut out. RT-PCR was used to detect interleukin-1(IL-1),interleukin-6(IL-6),tumor necrosis factorα(TNF-α),and interferon -γ(IFN-γ)expression in the temporomandibular joint cartilage. Results In occlusal interference condition,RT-PCR results showed inflammatory cytokines IL-1,IL-6 and TNF-α in rats of different groups were expressed. However,the inflammatory cytokines expression of the experimental group were higher than those of control group. IL-6 and TNF-α expression in occlusal disorder group were higher than those of removed occlusal disorder group. IFN-γ expression in three groups was not detected. Conclusion Under the occlusal interference condition,the expression of the inflammatory cytokines IL-1,IL-6 and TNF-α in the temporomandibular joint cartilage got enhancement. These results suggested that inflammatory cytokines and temporomandibular joint condylar remodeling in rats activity correlated.