CAJ | 학술논문

目的：应用RT-PCR技术检测pN0期非小细胞肺癌( NSCLC)患者淋巴结肺特异性X蛋白mRNA (Lunx mRNA)、基质金属蛋白酶-7mRNA(MMP-7mRNA)的表达情况，探讨其作为分子标记物对于微转移的诊断价值和临床意义。方法检测41例行根治术的pN0期非小细胞肺癌患者的171枚淋巴结中Lunx mR-NA、MMP-7 mRNA的表达。结果15例(36.6%)患者的33枚(19.3%)淋巴结检测存在微转移，这部分患者的TNM分期被重新调整。伴有淋巴结微转移的患者较无微转移的患者有较短的5年生存期( P=0.000)。多因素分析显示，淋巴结微转移、临床分期为影响NSCLC预后的独立危险因素。结论早期诊断区域淋巴结中常规病理检查难以发现的微转移对于NSCLC患者的分子分期、临床治疗和预后判断有着重要的意义。
목적：응용RT-PCR기술검측pN0기비소세포폐암( NSCLC)환자림파결폐특이성X단백mRNA (Lunx mRNA)、기질금속단백매-7mRNA(MMP-7mRNA)적표체정황，탐토기작위분자표기물대우미전이적진단개치화림상의의。방법검측41례행근치술적pN0기비소세포폐암환자적171매림파결중Lunx mR-NA、MMP-7 mRNA적표체。결과15례(36.6%)환자적33매(19.3%)림파결검측존재미전이，저부분환자적TNM분기피중신조정。반유림파결미전이적환자교무미전이적환자유교단적5년생존기( P=0.000)。다인소분석현시，림파결미전이、림상분기위영향NSCLC예후적독립위험인소。결론조기진단구역림파결중상규병리검사난이발현적미전이대우NSCLC환자적분자분기、림상치료화예후판단유착중요적의의。
Objective Using real time reverse transcriptase polymerase chain reaction ( RT-PCR) to detect expres-sion of Lunx mRNA and MMP-7 mRNA in lymph nodes from pN0 non-small cell lung cancer(NSCLC) patients, and to discuss its diagnostic value and clinical significance to micrometastasis. Method 171 lymph nodes obtained from 41 cases of pN0 NSCLC going curative operation were examined, and the expression of Lunx mRNA and MMP-7 mRNA were analyzed. Result Micrometastases were detected in 33 lymph nodes ( 19. 3%) from 15 patients (36. 6%). The TNM staging of these 15 patients was upregulated. The 5-year survival rate of patients with microme-tastasis was significantly lower than that of patients without micrometastasis (P=0. 000). The results of multivariate analysis confirmed that lymph node micrometastasis and clinical stage were independent prognostic factors. Conclu-sion Early diagnosis of regional lymph node micrometastases that routine pathologic examination is hard to find has important significance in molecular staging, clinical treatment and prognosis judgement for NSCLC patients.