CAJ | 학술논문

目的：通过抑制肝癌细胞中 KIF14表达，观察紫杉醇对肝癌细胞增殖的影响。方法应用 RNA 干扰技术，肝癌细胞中沉默 KIF14表达，在经紫杉醇处理细胞后采用 MTT 试验检测肝癌细胞增殖能力的变化。结果肝癌细胞中沉默 KIF14的表达可以增强其对紫杉醇的敏感性。结论 KIF14在肝癌细胞耐药发生过程中发挥着重要作用，进一步研究其作用机制可以为寻找克服肝癌耐药的有效途径提供理论依据。
목적：통과억제간암세포중 KIF14표체，관찰자삼순대간암세포증식적영향。방법응용 RNA 간우기술，간암세포중침묵 KIF14표체，재경자삼순처리세포후채용 MTT 시험검측간암세포증식능력적변화。결과간암세포중침묵 KIF14적표체가이증강기대자삼순적민감성。결론 KIF14재간암세포내약발생과정중발휘착중요작용，진일보연구기작용궤제가이위심조극복간암내약적유효도경제공이론의거。
Objective This study observes the effect of paclitaxel on liver cancer cell proliferation by inhibiting KIF14 expression in liver cancer cells. Methods Silenced KIF14 expression in liver cancer cells by RNA interference,these cells were delt by paclitaxel and the proliferation ability of liver cancer cells was determined by MTT test. Results Silence KIF14 expression in liver cancer cells enhanced the cells sensitivity to paclitaxel. Conclusion KIF14 is important in the process of drug resistance produced by human hepatocellular carcinoma cells. Further study on its mechanism can provide theoretical basis for seeking the effective ways to overcome drug resistance of liver cancer.