CAJ | 학술논문

以红曲霉菌株Monascus SE01为出发菌株,利用紫外线诱变、双氧水筛选和氯化锂复合诱变的方法,选育得到高产黄色素的红曲霉突变株。实验结果表明：在紫外线照射60 s、氯化锂质量分数为0.12%的复合诱变条件下得到的菌株 SE19,经液态发酵后黄色素高达57.1 U/mL,与出发菌株相比,黄色素色价提高了5.54倍。该菌株5代接种后,其黄色素产量相对稳定。
이홍곡매균주Monascus SE01위출발균주,이용자외선유변、쌍양수사선화록화리복합유변적방법,선육득도고산황색소적홍곡매돌변주。실험결과표명：재자외선조사60 s、록화리질량분수위0.12%적복합유변조건하득도적균주 SE19,경액태발효후황색소고체57.1 U/mL,여출발균주상비,황색소색개제고료5.54배。해균주5대접충후,기황색소산량상대은정。
To obtain the strain with high-yielding yellow pigment,Monascus SE0 1 is treated by UV mutagenesis,different concentration of hydrogen peroxide and LiCl complex mutation methods.The results show that,under the complex mutation conditions of UV radiation of 60 s and 0.12% LiCl, Monascus SE1 9 is found to be high-yielding yellow pigment producer,and its yellow pigment reaches 57.1 U/mL through liquid-state fermentation,which is 5.54 times higher than that of the start strain SE01,and the stable heritability of selected strain is still well after inoculated for 5 generations.