CAJ | 학술논문

目的：研究钙敏感受体(CaSR)在人骨性关节炎(OA)软骨中的表达强度，探讨其与OA发病机制的关系。方法收集5例因膝关节OA接受全膝关节置换术患者的股骨远端及胫骨近端标本，HE染色后根据Collins病理学分级标准进行关节软骨退变分级，Collins 0级切片8张为软骨正常组，CollinsⅡ级切片8张为软骨退变组，免疫组织化学染色后比较2组软骨组织内CaSR的表达强度。结果 CaSR在OA软骨中阳性表达，CaSR在软骨正常组的表达强度低于退变软骨组（评分：1.63±0.95 vs 3.52±0.78，t=8.99，P＜0.05）。结论 CaSR活化与关节软骨退变有关。
목적：연구개민감수체(CaSR)재인골성관절염(OA)연골중적표체강도，탐토기여OA발병궤제적관계。방법수집5례인슬관절OA접수전슬관절치환술환자적고골원단급경골근단표본，HE염색후근거Collins병이학분급표준진행관절연골퇴변분급，Collins 0급절편8장위연골정상조，CollinsⅡ급절편8장위연골퇴변조，면역조직화학염색후비교2조연골조직내CaSR적표체강도。결과 CaSR재OA연골중양성표체，CaSR재연골정상조적표체강도저우퇴변연골조（평분：1.63±0.95 vs 3.52±0.78，t=8.99，P＜0.05）。결론 CaSR활화여관절연골퇴변유관。
Objective To investigate calcium-sensing receptor (CaSR) expression level in osteoarthritis (OA) carti-lage,and the relationship with OA pathogenesis thereof. Methods The degenerated human cartilage of distal femur and proximal tibia came from 5 patients with OA underwent total knee replacement surgery. The tissue specimens were stained by HE. According to the Collins histological grading standards, articular cartilage degeneration was classificated in speci-mens. Eight HE slices of Collins grade 0 were used as normal cartilage group. Eight HE slices of Collins gradeⅡwere used as degeneration cartilage group. The CaSR expression levels detected by immunohistochemical staining technique were com-pared between two groups. Results Expression of CaSR was detected in osteoarthritis cartilage. The expression intensity of CaSR was significantly lower in normal cartilage group than that of degeneration of cartilage group (score:1.63±0.95 vs 3.52± 0.78,t=8.99,P<0.05). Conclusion The activation of CaSR is associated with the degeneration of articular cartilage.