CAJ | 학술논문

目的：探讨人参皂苷Rg1对脑缺血再灌注大鼠脑组织一氧化氮合酶（NOS）表达的影响。方法将30只大鼠随机分成假手术组、脑缺血再灌注组、Rg1-L组、Rg1-M组、Rg1-H组及尼莫地平组，每组5只。采用大鼠中动脉栓塞法制作大鼠脑缺血再灌注模型，观察大鼠再灌注后神经功能缺损情况，采用硝酸还原酶法和比色法检测大鼠血清中NO、神经型NOS（nNOS），诱导型NOS（iNOS）的含量，应用免疫组化和免疫印迹法检测脑缺血再灌注后nNOS、iNOS的表达。结果（1）Rg1-L组、Rg1-M组、Rg1-H组大鼠的脑缺血后神经功能评分明显低于脑缺血再灌注组（2.40±0.55、1.80±0.84、1.60±0.89 vs 3.20±0.84，P<0.05）。（2）与脑缺血再灌注组相比，3个Rg1组大鼠血清中NO和iNOS含量降低，nNOS含量增高。（3）与脑缺血再灌注组相比，3个Rg1组大鼠脑组织中nNOS表达增高，iNOS表达降低。结论人参皂苷Rg1防治大鼠脑缺血再灌注损伤的机制可能与激活nNOS、抑制iNOS有关。
목적：탐토인삼조감Rg1대뇌결혈재관주대서뇌조직일양화담합매（NOS）표체적영향。방법장30지대서수궤분성가수술조、뇌결혈재관주조、Rg1-L조、Rg1-M조、Rg1-H조급니막지평조，매조5지。채용대서중동맥전새법제작대서뇌결혈재관주모형，관찰대서재관주후신경공능결손정황，채용초산환원매법화비색법검측대서혈청중NO、신경형NOS（nNOS），유도형NOS（iNOS）적함량，응용면역조화화면역인적법검측뇌결혈재관주후nNOS、iNOS적표체。결과（1）Rg1-L조、Rg1-M조、Rg1-H조대서적뇌결혈후신경공능평분명현저우뇌결혈재관주조（2.40±0.55、1.80±0.84、1.60±0.89 vs 3.20±0.84，P<0.05）。（2）여뇌결혈재관주조상비，3개Rg1조대서혈청중NO화iNOS함량강저，nNOS함량증고。（3）여뇌결혈재관주조상비，3개Rg1조대서뇌조직중nNOS표체증고，iNOS표체강저。결론인삼조감Rg1방치대서뇌결혈재관주손상적궤제가능여격활nNOS、억제iNOS유관。
Objective To investigate the effects of Ginsenoside Rg1 on the expression of nitric oxide synthase (NOS) in brain tissue after cerebral arterial thrombosis in adult rats. Methods Thirty rats were randomly divided into the sham-operative group, cerebral ischemia-reperfusion group, Ginsenoside Rg1-L group, Ginsenoside Rg1-M group, Ginsen-oside Rg1-H group and nimodipine group (n=5 for each group). The ischemia-reperfusion rat model was established by mid-dle cerebral artery occlusion. The neurological score after reperfusion was observed. The levels of nitric oxide (NO), neuronal NOS (nNOS) and inducible NOS (iNOS) were detected by nitrate reduction method and colorimetric method. The expressions of nNOS and iNOS after reperfusion were analyzed by immunohistochemistry and Western blot assay. Results (1) The neu-rological scores after cerebral ischemia were significantly lower in Rg1-L group, Rg1-M group and Rg1-H group than those of cerebral ischemia-reperfusion group(2.40±0.55,1.80±0.84, 1.60±0.89 vs 3.20±0.84,P<0.05). (2) Compared with those of model group, serum levels of NO and iNOS were reduced, and nNOS levels increased, in three groups of Rg1. (3) Compared with those of model group, the expression of nNOS was significantly increased,and iNOS expression was significantly re-duced, in three groups of Rg1. Conclusion The preventive effects of Ginsenosides Rg1 on cerebral ischemia-reperfusion injury may be associated with the activation of nNOS and the inhibition of iNOS.