CAJ | 학술논문

目的：探讨人肺癌细胞受照后γH2AX表达量变化与肺癌细胞放射敏感性之间的关系。方法选择肺腺癌A549和小细胞肺癌SBC-3细胞株，采用细胞克隆形成实验检测经不同剂量（0、2、4、6、8、10 Gy）照射后A549和SBC-3细胞的克隆形成率，并绘制细胞生存曲线；应用Western blot检测经2 Gy放射剂量照射后不同时间点（0 min、30 min、1 h、2 h、6 h、12 h、24 h、36 h、48 h）的细胞中γH2AX蛋白表达量。分析γH2AX蛋白表达量变化与肿瘤细胞放射敏感性的相关性。结果小细胞肺癌SBC-3细胞的放射敏感性明显高于肺腺癌A549细胞。两种细胞受照后1 h和6 h，γH2AX的表达量均与肿瘤细胞的平均致死剂量（D0）相关。结论γH2AX可以作为肺癌细胞放射敏感性的一个预测指标。
목적：탐토인폐암세포수조후γH2AX표체량변화여폐암세포방사민감성지간적관계。방법선택폐선암A549화소세포폐암SBC-3세포주，채용세포극륭형성실험검측경불동제량（0、2、4、6、8、10 Gy）조사후A549화SBC-3세포적극륭형성솔，병회제세포생존곡선；응용Western blot검측경2 Gy방사제량조사후불동시간점（0 min、30 min、1 h、2 h、6 h、12 h、24 h、36 h、48 h）적세포중γH2AX단백표체량。분석γH2AX단백표체량변화여종류세포방사민감성적상관성。결과소세포폐암SBC-3세포적방사민감성명현고우폐선암A549세포。량충세포수조후1 h화6 h，γH2AX적표체량균여종류세포적평균치사제량（D0）상관。결론γH2AX가이작위폐암세포방사민감성적일개예측지표。
Objective To observe the relationship between expression changes of γH2AX and the radiosensitivity of lung cancer cells in vitro. Methods The radiosensitivity of lung cancer cell lines A549 and SBC-3 was measured by clone forming assay. The DSBs damage of lung cancer cell lines A549 and SBC-3 was determined by Western blot assay. Re-sults The clone forming rates of lung cancer cell lines A549 and SBC-3 were gradually decreased with the increased radia-tion dose.γH2AX expression was related to the cell radiosensitivity 1 hour and 6 hours after radiated. Conclusion The phosphorylated histoneγH2AX is a powerful tool to monitor DNA DSBs and to predict the radiosensitivity in lung cancer ra-diotherapy.