CAJ | 학술논문

杆状病毒表面展示系统（Baculovirus Surface Display，BSD）是近年来发展起来的一种崭新的膜展示技术。其主要原理是通过基因工程的方法，将杆状病毒表面囊膜蛋白基因与所要展示的目的蛋白基因进行融合，形成重组杆状病毒。重组杆状病毒的囊膜蛋白在表达同时，目的蛋白也随即进行表达，并与囊膜蛋白共同运输至病毒囊膜处，特异的与锚定部位结合，展示在杆状病毒表面。该系统由于具有较强的可控性和较高的展示效率，目前已经被广泛应用于基因转导与基因治疗、较复杂的真核蛋白展示、新型疫苗研发以及单克隆抗体的制备等领域。本文对杆状病毒表面展示系统的研究现状进行了阐述和总结，并对其发展和应用前景做出了展望。
간상병독표면전시계통（Baculovirus Surface Display，BSD）시근년래발전기래적일충참신적막전시기술。기주요원리시통과기인공정적방법，장간상병독표면낭막단백기인여소요전시적목적단백기인진행융합，형성중조간상병독。중조간상병독적낭막단백재표체동시，목적단백야수즉진행표체，병여낭막단백공동운수지병독낭막처，특이적여묘정부위결합，전시재간상병독표면。해계통유우구유교강적가공성화교고적전시효솔，목전이경피엄범응용우기인전도여기인치료、교복잡적진핵단백전시、신형역묘연발이급단극륭항체적제비등영역。본문대간상병독표면전시계통적연구현상진행료천술화총결，병대기발전화응용전경주출료전망。
Successful examples in Baculovirus Surface Display system (BSD) have recently been achieved by the recombinant DNA technique via recombinant DNA technology. The main principle is to combine the bac-ulovirus surface envelope protein genes and the target genes and to express a fusion protein via the recombinant baculovirus. The envelope protein of recombinant baculovirus is expressed, meanwhile, the target protein is expressed as well. The target genes are expressed and transported to virus envelope with the envelope protein, then specifically anchored and displayed on the baculovirus surface. Due to the strong controllability and high efficiency, BSD has been widely used in gene transduction, gene therapy, complex eukaryotic proteins dis-play, new vaccine development and the preparation of monoclonal antibodies, etc. In this paper, the re-search status quo of baculovirus surface display system is reviewed, including the development and application prospect.