CAJ | 학술논문

代谢网络在代谢功能研究、生物代谢过程控制、疾病诊断分析和药物靶标设计等方面具有重要理论和实践意义。生物信息学研究利用序列同源、结构模拟、对接等手段与生化实验有效结合促进了生物体代谢网络的进一步完善。本文作者在构建幽门螺杆菌（Helicobacter pylori 26695，H．pylori 26695）代谢网络的工作基础上综合了近年来研究者对H．pylori 26695代谢通路关键酶的研究成果，并结合基因组信息，综述了H．pylori 26695特异性的重要代谢通路。本文从基因组水平阐明代谢通路与基因的关系，并详细分析了关键酶对H．pylori 26695生理的重要作用，最后探讨了重构一个连续、完整的代谢网络面临的困难及其在药物靶标设计方面的研究前景。
대사망락재대사공능연구、생물대사과정공제、질병진단분석화약물파표설계등방면구유중요이론화실천의의。생물신식학연구이용서렬동원、결구모의、대접등수단여생화실험유효결합촉진료생물체대사망락적진일보완선。본문작자재구건유문라간균（Helicobacter pylori 26695，H．pylori 26695）대사망락적공작기출상종합료근년래연구자대H．pylori 26695대사통로관건매적연구성과，병결합기인조신식，종술료H．pylori 26695특이성적중요대사통로。본문종기인조수평천명대사통로여기인적관계，병상세분석료관건매대H．pylori 26695생리적중요작용，최후탐토료중구일개련속、완정적대사망락면림적곤난급기재약물파표설계방면적연구전경。
Metabolic network is widely used in metabolic studies, biological metabolic process control, disease diagnosis and drug target design. Bioinformatics studies are now combined with biochemical experiments effectively by the means of sequence homologous, structure simulation and docking to promote the completion of organism metabolic network. Based on the knowledge of genome-scale metabolic network reconstruction for H. pylori, we reviewed several important metabolic pathways by combining the recent achievements on key enzyme researches of Helicobacter pylori 26695 (H.pylori) as well as genome information.We revealed the relationship between genes and metabolic pathway and analyzed the vital functions of key enzymes in H. pylori. Lastly, the challenges in reconstructing a coherent, complete metabolic network are introduced in this paper and the perspectives in drug target designing are also discussed.