CAJ | 학술논문

目的：明确我国大陆地区基因1a亚型丙型肝炎病毒系统进化的情况。方法收集慢性丙型肝炎患者的血清标本，采用逆转录-巢式PCR扩增HCV基因组部分NS5B区和（或）部分核心/E1区片段。根据测序结果构建系统进化树，分析大陆地区基因1a亚型丙型肝炎病毒系统进化情况。结果共收集全国23个中心共1012例患者标本，其中970例标本分型成功，基因1a亚型患者4例。以美国国立卫生院丙型肝炎数据库中不同国家来源丙型肝炎病毒序列为参考构建系统进化树成功。系统进化分析提示，4株HCV 1a亚型部分NS5B区序列散在分布于进化树中的不同分支，来源于不同国家。结论基因1a型HCV由国外传播入我国，在中国丙型肝炎病毒感染者中所占比率低，在大陆地区随机流行。
목적：명학아국대륙지구기인1a아형병형간염병독계통진화적정황。방법수집만성병형간염환자적혈청표본，채용역전록-소식PCR확증HCV기인조부분NS5B구화（혹）부분핵심/E1구편단。근거측서결과구건계통진화수，분석대륙지구기인1a아형병형간염병독계통진화정황。결과공수집전국23개중심공1012례환자표본，기중970례표본분형성공，기인1a아형환자4례。이미국국립위생원병형간염수거고중불동국가래원병형간염병독서렬위삼고구건계통진화수성공。계통진화분석제시，4주HCV 1a아형부분NS5B구서렬산재분포우진화수중적불동분지，래원우불동국가。결론기인1a형HCV유국외전파입아국，재중국병형간염병독감염자중소점비솔저，재대륙지구수궤류행。
Objective To investigate the phylogeny proifle of subgenotype 1a of hepatitis C virus in mainland China. Methods Serum samples and clinical data were collected from patients with chronic hepatitis C from 23 sites in mainland China. NS5B and/or Core/E1 region for sequencing were amplified by RT-nested PCR. Phylogenetic tree which built with reference sequences from NIH HCV database and phylogeny proifle of subgenotype 1a of hepatitis C virus in mainland China were analyzed. Results Serum sample and clinical data were collected from 1 012 enrolled patients with CHC. HCV subgenotype were identiifed in 970/1 012 patients. Total of 4 patients with subgenotype 1a were identiifed and phylogenetic tree were built with reference sequence for NIH HCV database. Phylogenetic analysis showed these 4 sugenotype 1a NS5B region sequences originated from different countries and scattered in different branches in the phylogenetic tree. Conclusions Subgenotype 1a of HCV in mainland China originated from abroad. The proportion of subgenotype 1a in all Chinese HCV subgenotypes is low and subgenotype 1a patients are scattered in mainland China, randomly.