中国药物评价
中國藥物評價
중국약물평개
Chinese Journal of Drug Evaluation
2014年
4期
199-201,214
,共4页
朱海芳%海娟%王春艳%李民%郑海荣%段桂峰
硃海芳%海娟%王春豔%李民%鄭海榮%段桂峰
주해방%해연%왕춘염%리민%정해영%단계봉
桑叶枇杷阿胶膏%高效液相色谱法%芦丁
桑葉枇杷阿膠膏%高效液相色譜法%蘆丁
상협비파아효고%고효액상색보법%호정
Sangye Pipa Ejiao paste%High performance liquid chromatography ( HPLC)%Rutin
目的：建立胶类复方制剂桑叶枇杷阿胶膏中芦丁的高效液相色谱法（ HPLC）含量测定方法。方法：色谱柱为Waters SunFire C18柱（4.6 mm ×250 mm，5μm）；流动相为甲醇-0.5％磷酸溶液（梯度洗脱）；流速为1.0 mL· min-1；检测波长为358 nm。结果：芦丁在0.1025～2.0500μg范围内与其峰面积呈良好线性关系（ r＝0.9999），平均加样回收率为98.63％， RSD为2.27％。结论：该测定方法简单易行，结果准确，重复性好，可用于测定桑叶枇杷阿胶膏中芦丁含量。
目的：建立膠類複方製劑桑葉枇杷阿膠膏中蘆丁的高效液相色譜法（ HPLC）含量測定方法。方法：色譜柱為Waters SunFire C18柱（4.6 mm ×250 mm，5μm）；流動相為甲醇-0.5％燐痠溶液（梯度洗脫）；流速為1.0 mL· min-1；檢測波長為358 nm。結果：蘆丁在0.1025～2.0500μg範圍內與其峰麵積呈良好線性關繫（ r＝0.9999），平均加樣迴收率為98.63％， RSD為2.27％。結論：該測定方法簡單易行，結果準確，重複性好，可用于測定桑葉枇杷阿膠膏中蘆丁含量。
목적：건립효류복방제제상협비파아효고중호정적고효액상색보법（ HPLC）함량측정방법。방법：색보주위Waters SunFire C18주（4.6 mm ×250 mm，5μm）；류동상위갑순-0.5％린산용액（제도세탈）；류속위1.0 mL· min-1；검측파장위358 nm。결과：호정재0.1025～2.0500μg범위내여기봉면적정량호선성관계（ r＝0.9999），평균가양회수솔위98.63％， RSD위2.27％。결론：해측정방법간단역행，결과준학，중복성호，가용우측정상협비파아효고중호정함량。
Objective:To establish the high performance liquid chromatography ( HPLC) method for determination the content of rutin in Sangye Pipa Ejiao paste.Methods:Waters SunFire C18 column (4.6 mm ×250 mm,5 μm) column was used with the mobile phase composed of methanol-phosphoric acid solution (0.5%) (gradient elute) at the flow rate of 1.0 mL· min-1.The detection wavelength was 358 nm.Results: Rutin showed a good linear relationship to the peak aera at a concentration rang of 0.1025~2.0500 μg ( r=0.9999).The average recovery was 98.63%, with RSD of 2.27%.Conclusion: The method is accurate, reliable, sensitive, simple and suitable for the determination of content of rutin in Sangye Pipa Ejiao paste.
