CAJ | 학술논문

Tbx5参与转录因子的调控,对上肢及心脏发育起关键作用.通过对小鼠胚胎心脏组织切片厚度的选择、加入抗原修复液等试验条件的优化,建立了利用切片免疫荧光技术检测小鼠胚胎心脏中Tbx5的方法.结果显示,在胚胎发育的第11.5 d, Tbx5在整个心脏中都有表达,尤其在心房以及左心室中高表达.但在胚胎发育的第13.5 d, Tbx5只在心房和左心室中表达,在右心室中并不表达.揭示Tbx5的这种动态表达模式对于心脏的发育以及腔室分化是十分重要的.
Tbx5삼여전록인자적조공,대상지급심장발육기관건작용.통과대소서배태심장조직절편후도적선택、가입항원수복액등시험조건적우화,건립료이용절편면역형광기술검측소서배태심장중Tbx5적방법.결과현시,재배태발육적제11.5 d, Tbx5재정개심장중도유표체,우기재심방이급좌심실중고표체.단재배태발육적제13.5 d, Tbx5지재심방화좌심실중표체,재우심실중병불표체.게시Tbx5적저충동태표체모식대우심장적발육이급강실분화시십분중요적.
The transcriptional regulation factor.Tbx5 played a key role on the upper limbs and heart development. Special Immunofluorescence was effectively established for the expression of Tbx5 in embryonic heart, which was optimized through a series of trials such as the selection of tissular slice thickness, adding the antigen repair solution. The results showed Tbx5 was expressed in the heart in 11.5 dpc mouse, especially highly expressed in the atrium and the left ventricle. At E13.5, Tbx5 was expressed in the atrium and left ventricular, was’t expressed in the right ventricular. Indicating the dynamic expression pattern of Tbx5 was important for the development of heart and the differentiation of Chambers.