CAJ | 학술논문

研究旨在观察亮甲酚蓝（brilliant cresyl blue，BCB）对牛卵母细胞的染色效果及体外成熟的影响，从而筛选出更高质量的卵母细胞用于胚胎工程研究。从本地屠宰场收集牛卵巢后采用抽吸法获取卵泡液，在体视显微镜下选择卵丘完整、胞质均匀的卵丘-卵母细胞复合体（ COCs ）随机分组，分别进行BCB染色时间和不同浓度BCB染色的试验。结果表明，染色90 min后卵母细胞着色率为68．1％，显著高于30、60 min组（ P＜0．05），辨识难度为“中”；39μmol/L 组着色率显著高于13μmol/L 组（ P＜0．05），与26、52μmol/L 组差异不显著（P＞0．05），成熟数最高（62），成熟率显著高于52μmol/L组，与其它组差异不显著（P＞0．05）；BCB＋组和BCB-组的成熟率与对照组比较差异均不显著（ P＞0．05）。结果提示，39μmol/L BCB染色90 min能有效筛选牛COCs用于IVM，但是并不能显著提高牛卵母细胞的成熟率。
연구지재관찰량갑분람（brilliant cresyl blue，BCB）대우란모세포적염색효과급체외성숙적영향，종이사선출경고질량적란모세포용우배태공정연구。종본지도재장수집우란소후채용추흡법획취란포액，재체시현미경하선택란구완정、포질균균적란구-란모세포복합체（ COCs ）수궤분조，분별진행BCB염색시간화불동농도BCB염색적시험。결과표명，염색90 min후란모세포착색솔위68．1％，현저고우30、60 min조（ P＜0．05），변식난도위“중”；39μmol/L 조착색솔현저고우13μmol/L 조（ P＜0．05），여26、52μmol/L 조차이불현저（P＞0．05），성숙수최고（62），성숙솔현저고우52μmol/L조，여기타조차이불현저（P＞0．05）；BCB＋조화BCB-조적성숙솔여대조조비교차이균불현저（ P＞0．05）。결과제시，39μmol/L BCB염색90 min능유효사선우COCs용우IVM，단시병불능현저제고우란모세포적성숙솔。
This experiment aimed at the observation of the staining effect of brilliant cresyl blue ( BCB ) and its influence on bovine oocyte in vitro maturation so as to select the higher quality oocytes for the embryo engineering research .Bovine ovaries were collected from a local slaughterhouse , and with the suction method the follicular fluid was obtained ,cumulus oocyte complexes ( COCs) with intact cumulus,homogeneous cyto-plasm were selected by using a stereoscopic microscope ,and then were randomly divided into two groups ,and the staining time and BCB concentration were determined , respectively .The results showed that , the staining rate of oocyte 90 min after staining was 68.1%,it was significantly higher than that of the groups of 30 and 60 min,and the distinguishable difficulty was “common”.The rate of stained oocytes in the 39μmol/L group was significantly higher than that in the 13μmol/L group ( P<0.05) ,and was not different from that in the 26 and 52 μmol/L groups (P>0.05),the No.of mature oocytes was the highest (62),the maturation rate was signifi-cantly higher than that in the 52μmol/L group (P<0.05),and was not different from that in the other groups ( P>0.05) .The maturation rates of the BCB +group and BCB-group were not significantly different from that of the control group ( P>0.05) .These results suggest that ,staining with 39μmol/L of BCB for 90 min is effective for selection of bovine COCs for IVM ,but has no significant efficiency in improving the rate of bovine oocyte in vitro maturation .