江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2014年
4期
776-781
,共6页
吴道慧%张余杰%董丁铭%秦小萍%刘嘉羚
吳道慧%張餘傑%董丁銘%秦小萍%劉嘉羚
오도혜%장여걸%동정명%진소평%류가령
坡柳%皂苷%粘虫%拒食活性%发育抑制
坡柳%皂苷%粘蟲%拒食活性%髮育抑製
파류%조감%점충%거식활성%발육억제
Dodonaea viscosa%saponin%Mythimna separate%antifeedant activity%growth inhibition
研究了坡柳皂苷对粘虫生长发育的活性。采用浸渍叶碟法，分别测试了5个质量分数处理对粘虫的生物活性。结果表明：坡柳皂苷对粘虫具有较好的发育抑制，且随浓度的增大而提高。24 h后2000，3000，4000，5000，6000μg/mL坡柳皂苷处理对粘虫4龄幼虫的发育抑制率分别为24．36％、37．81％、49．38％、54．93％、71．30％，48 h后为15．14％、44．91％、58．66％、61．42％、68．74％；上述5种处理24 h后的拒食率分别为8．72％、26．74％、41．53％、55．92％、64．39％，处理48 h后拒食率分别为4．08％、30．18％、57．66％、60．14％、68．03％；13 d后，6000μg/mL处理幼虫的化蛹率为32％，显著低于CK （92％）；上述5种处理24 d后蛹的羽化率分别为56％、48％、36％、32％、20％；40 d后上述5种处理卵的孵化率分别为36．74％、23．61％、7．76％、5．57％、5．46％。因此，坡柳皂苷对粘虫幼虫的生长发育、取食、化蛹和蛹的羽化、卵的孵化具有一定程度的抑制效果。
研究瞭坡柳皂苷對粘蟲生長髮育的活性。採用浸漬葉碟法，分彆測試瞭5箇質量分數處理對粘蟲的生物活性。結果錶明：坡柳皂苷對粘蟲具有較好的髮育抑製，且隨濃度的增大而提高。24 h後2000，3000，4000，5000，6000μg/mL坡柳皂苷處理對粘蟲4齡幼蟲的髮育抑製率分彆為24．36％、37．81％、49．38％、54．93％、71．30％，48 h後為15．14％、44．91％、58．66％、61．42％、68．74％；上述5種處理24 h後的拒食率分彆為8．72％、26．74％、41．53％、55．92％、64．39％，處理48 h後拒食率分彆為4．08％、30．18％、57．66％、60．14％、68．03％；13 d後，6000μg/mL處理幼蟲的化蛹率為32％，顯著低于CK （92％）；上述5種處理24 d後蛹的羽化率分彆為56％、48％、36％、32％、20％；40 d後上述5種處理卵的孵化率分彆為36．74％、23．61％、7．76％、5．57％、5．46％。因此，坡柳皂苷對粘蟲幼蟲的生長髮育、取食、化蛹和蛹的羽化、卵的孵化具有一定程度的抑製效果。
연구료파류조감대점충생장발육적활성。채용침지협설법，분별측시료5개질량분수처리대점충적생물활성。결과표명：파류조감대점충구유교호적발육억제，차수농도적증대이제고。24 h후2000，3000，4000，5000，6000μg/mL파류조감처리대점충4령유충적발육억제솔분별위24．36％、37．81％、49．38％、54．93％、71．30％，48 h후위15．14％、44．91％、58．66％、61．42％、68．74％；상술5충처리24 h후적거식솔분별위8．72％、26．74％、41．53％、55．92％、64．39％，처리48 h후거식솔분별위4．08％、30．18％、57．66％、60．14％、68．03％；13 d후，6000μg/mL처리유충적화용솔위32％，현저저우CK （92％）；상술5충처리24 d후용적우화솔분별위56％、48％、36％、32％、20％；40 d후상술5충처리란적부화솔분별위36．74％、23．61％、7．76％、5．57％、5．46％。인차，파류조감대점충유충적생장발육、취식、화용화용적우화、란적부화구유일정정도적억제효과。
The biological activity of Dodonaea viscosa saponin on the growth and development of Mythimna separate were determined .5 concentrations were tested by leaf dish dip method .The results showed that:Dodonaea viscosa saponin had good inhibition on the development of Mythimna separate and the inhibition in-creased with concentration .The growth inhibition rates of 2 000,3 000,4 000,5 000 and 6 000 μg/mL treat-ments were 24.36%,37.81%,49.38%,54.93%,71.30%respectively in 24 h,15.14%,44.91%,58.66%, 61.42%,68.74%in 48 h;after 24 h,the antifeedant rates of the above 5 treatments were 8.72%,26.74%, 41.53%,55.92%,64.39%respectively and 4.08%,30.18%,57.66%,60.14%,68.03%respectively in 48 h;in 13 d,the pupation inhibition rate of the 6 000 μg/mL treatment was 32%,significantly lower than that of the CK(92%);the eclosion rates of the above 5 treatments were 68%,56%,48%,36%,32%,20%respectively in 24 d;after 40 d,the egg hatching rates of the above 5 treatments were 36.74%,23.61%,7.76%,5.57%,5.46%respectively.Therefore,Dodonaea viscosa saponin has inhibitory effect on the growth ,feeding,pupation,emer-gence and egg hatching of Mythimna separate.
