CAJ | 학술논문

在对杉木人工林林下植被调查研究的基础上，通过对根瘤菌进行分离与鉴定，筛选出林下固氮植被，以期为杉木人工林林地功能菌菌肥的研究提供技术支持。对植物的根瘤菌进行研究，分离得到7株菌株，用乙炔还原法对根瘤样品进行固氮酶活性测定。其中菌株YG-1和菌株LYX-3的固氮酶活性较高，分别为134．8μmol C2H4/（g· h）和115．6μmol C2H4/（g· h）。通过菌株的生理生化实验及回接实验将菌株YG-1、LYX-3初步鉴定为根瘤菌属。筛选出的具有固氮能力的植物2种，分别是野葛和六月雪。
재대삼목인공림림하식피조사연구적기출상，통과대근류균진행분리여감정，사선출림하고담식피，이기위삼목인공림임지공능균균비적연구제공기술지지。대식물적근류균진행연구，분리득도7주균주，용을결환원법대근류양품진행고담매활성측정。기중균주YG-1화균주LYX-3적고담매활성교고，분별위134．8μmol C2H4/（g· h）화115．6μmol C2H4/（g· h）。통과균주적생리생화실험급회접실험장균주YG-1、LYX-3초보감정위근류균속。사선출적구유고담능력적식물2충，분별시야갈화륙월설。
Based on the research of the diversity of vegetation ,rhizobium samples were isolated and identi-fied to screen the nitrogen-fixing vegetation under Cunninghamia lanceolata Plantation so as to support future development of functional bacteria manure .Through studying the rhizobium samples ,7 strains of YG-1,LYX-3 were obtained and their nitrogenase activity was tested .The result showed that the strains of YG-1 and LYX-3 had higher nitrogenase activity making 134.8 μmol C2 H4/g· h and 115.6 μmol C2 H4/g· h,respectively. They were identified as Rhizobium by preliminary identification and back inoculation experiment .Two nitrogen-fixing plants were screened ,which are Pueraria lobata and Serissafoetida.