CAJ | 학술논문

通过追踪测定黄菖蒲叶绿素a、丙二醛（MDA）含量及超氧化物歧化酶（SOD）、过氧化物酶（POD）活性，研究了不同浓度NH4+对黄菖蒲抗氧化酶系统的影响。结果表明，NH4+质量浓度为0.5和1 mg/L时，黄菖蒲各理化指标变化平缓，0.5～1 mg/L是黄菖蒲生长的适宜的NH4+质量浓度；而在ρ（NH4+）≥3 mg/L时，NH4+对黄菖蒲产生了明显的胁迫作用，表现为在培养过程中，黄菖蒲叶绿素a含量逐渐降低，SOD及POD活性呈现先增加后逐渐降低的趋势，MDA含量逐渐增加，表明高浓度NH4+在经过一段时间的胁迫后，能够显著抑制黄菖蒲SOD及POD的活性，引起细胞的氧化损伤，并能促进叶绿素的分解，从而影响黄菖蒲的生长。
통과추종측정황창포협록소a、병이철（MDA）함량급초양화물기화매（SOD）、과양화물매（POD）활성，연구료불동농도NH4+대황창포항양화매계통적영향。결과표명，NH4+질량농도위0.5화1 mg/L시，황창포각이화지표변화평완，0.5～1 mg/L시황창포생장적괄의적NH4+질량농도；이재ρ（NH4+）≥3 mg/L시，NH4+대황창포산생료명현적협박작용，표현위재배양과정중，황창포협록소a함량축점강저，SOD급POD활성정현선증가후축점강저적추세，MDA함량축점증가，표명고농도NH4+재경과일단시간적협박후，능구현저억제황창포SOD급POD적활성，인기세포적양화손상，병능촉진협록소적분해，종이영향황창포적생장。
The effects of d ifferent concentrations of NH4+ on antioxidant system of Iris pseudacorus L. was studied by determining the chlorophyll-a content, MDA content, SOD and POD activities of Iris pseudacorus L.. Results showed that the growth of Iris pseudacorus L. was fitting at 0.5 and 1 mg/L concentrations of NH4+, and the physical and chemical indicators relatively mildly changed. But when NH4+ concentration was more than 3 mg/L, it had strong stress on Iris pseudacorus L.. Chlorophyll-a content of Iris pseudacorus L. declined gradually from the beginning, and MDA content of Iris pseudacorus L. increased gradually from the beginning, whereas SOD and POD activities increased first, but decreased as treatment time went on, indicating that the high concentration of NH4+ inhibited SOD and POD activity after a long incubation, caused oxidative damage to cells and promoted the degrading of chlorophyll of Iris pseudacorus L. which might be one of its mechanisms of restraining Iris pseudacorus L..