哈尔滨商业大学学报（自然科学版）
哈爾濱商業大學學報（自然科學版）
합이빈상업대학학보（자연과학판）
JOURNAL OF HARBIN UNIVERSITY OF COMMERCE(NATURAL SCIENCES EDITION)
2014年
4期
398-401
,共4页
八珍汤%芍药苷%质量浓度测定
八珍湯%芍藥苷%質量濃度測定
팔진탕%작약감%질량농도측정
Bazhen decoction%paecniflorin%content determination
建立八珍汤中芍药苷的高效液相色谱定量方法．HPLC分析采用Symmetry C18色谱柱（4．6 mm ×250 mm，5μm）；紫外检测器，检测波长为230 nm；流动相为乙腈-0．05％磷酸水溶液（15∶85）；流速为1．0 mL/min；柱温为30℃；进样量为20μL．芍药苷在0．014～0．224 mg/mL范围内呈良好线性关系，线性回归方程为Y ＝40798281 X ＋2855，r ＝0．9998（n＝5）．该方法具有分析速度快，重现性好，精密度高，定量准确等特点．适用于八珍汤复方中芍药苷的定量分析．
建立八珍湯中芍藥苷的高效液相色譜定量方法．HPLC分析採用Symmetry C18色譜柱（4．6 mm ×250 mm，5μm）；紫外檢測器，檢測波長為230 nm；流動相為乙腈-0．05％燐痠水溶液（15∶85）；流速為1．0 mL/min；柱溫為30℃；進樣量為20μL．芍藥苷在0．014～0．224 mg/mL範圍內呈良好線性關繫，線性迴歸方程為Y ＝40798281 X ＋2855，r ＝0．9998（n＝5）．該方法具有分析速度快，重現性好，精密度高，定量準確等特點．適用于八珍湯複方中芍藥苷的定量分析．
건립팔진탕중작약감적고효액상색보정량방법．HPLC분석채용Symmetry C18색보주（4．6 mm ×250 mm，5μm）；자외검측기，검측파장위230 nm；류동상위을정-0．05％린산수용액（15∶85）；류속위1．0 mL/min；주온위30℃；진양량위20μL．작약감재0．014～0．224 mg/mL범위내정량호선성관계，선성회귀방정위Y ＝40798281 X ＋2855，r ＝0．9998（n＝5）．해방법구유분석속도쾌，중현성호，정밀도고，정량준학등특점．괄용우팔진탕복방중작약감적정량분석．
To establish an HPLC method for determination of paecniflorin of Bazhen decoction the assay was performed on a Symmetry C 18 column (250 mm ×4.6 mm, 5μm).UV detec-tor wavelength was at 230 nm.The mobile phase was acetonitrile -0.05%phosphoric acid solution (15∶85).The flow rate was 1.0 mL/min, and the column temperature was main-tained at 30 ℃.The sampling volume was 20μL.The calibration curve had good linear re-lationship in the range of 0.014~0.224 mg/mL, linear equation: Y =40 798 281 X +2 855(r=0.999 8).This method was simple, sensitive, accurate and reproducible .It was suitable for quality control of Bazhen decoction .
