CAJ | 학술논문

薄层色谱分离效果的好坏，很大程度上取决于展层剂的组成和配比，还会受硅胶板的活化度、环境温度、显色剂和点样量手法等因素的影响。本文报道了采用薄层色谱法分析玉米芯降解液中单糖的成分，着重对多种展层剂进行优化研究。研究结果显示以正丁醇∶乙酸乙酯∶水＝7∶2∶1为展层剂分离糖的效果较好，本文还显示以苯胺-二苯胺-磷酸为显色剂的糖显色效果较好。同时，用上述优化的方法对不同酸浓度处理的玉米芯降解液进行分析研究，可以准确、清晰、便利地定性检测出玉米芯木聚糖中单糖的组分。
박층색보분리효과적호배，흔대정도상취결우전층제적조성화배비，환회수규효판적활화도、배경온도、현색제화점양량수법등인소적영향。본문보도료채용박층색보법분석옥미심강해액중단당적성분，착중대다충전층제진행우화연구。연구결과현시이정정순∶을산을지∶수＝7∶2∶1위전층제분리당적효과교호，본문환현시이분알-이분알-린산위현색제적당현색효과교호。동시，용상술우화적방법대불동산농도처리적옥미심강해액진행분석연구，가이준학、청석、편리지정성검측출옥미심목취당중단당적조분。
Effect of thin -layer chromatography mainly depends on the compose and proportion of developing solvents , as well influenced by silica gel plate activation grade ,environment temperature , color developing agentand and spotting technique .This paper reports that thin -layer chromatography analyze monosaccharide ingredients of the corncob degradation solution , emphasizing optimization study on developing solvent of the method .The result shows that thin -layer chromatography can be effective to separate the saccharides by Vn-butanol∶Vethyl acetate∶VWater =7∶2∶1 as developing solvent .The result also shows saccharides has better color effect by aniline -diphenylamine-phosphoric acid as chromogenic reagent .Meanwhile , The method of the optimization above analyzes degradation solution of different acid concentrations treatment corncob , which can effectively determination of the type of saccharides.Thus, the method can be accurate, clear, convenient qualitative detection of corncob xylan monosaccharide components .