CAJ | 학술논문

目的：比较鲍曼不动杆菌的分子生物学鉴定结果和仪器鉴定结果。方法采用法国梅里埃公司VITEK2 COMPACT全自动微生物分析仪对细菌进行鉴定和抗菌药物敏感性分析,鉴定为鲍曼不动杆菌复合群的细菌,采用聚合酶链反应( PCR)检测OXA-51基因。结果68株VITEK2 COMPACT鉴定为鲍曼不动杆菌复合群的菌株中,50株OXA-51基因阳性,确定为鲍曼不动杆菌；18株OXA-51基因阴性,为复合群其它细菌。鲍曼不动杆菌( OXA-51阳性)与复合群其它细菌( OXA-51阴性)比较,对临床常用的碳青酶烯类、氨基糖苷类、氟喹诺酮类、头孢菌素类等抗菌药物的敏感性降低(P<0．05)。结论鲍曼不动杆菌复合群其它细菌耐药率显著低于鲍曼不动杆菌,采用检测OXA-51基因方法对鲍曼不动杆菌复合群进行准确的菌种鉴定有利于正确指导临床抗菌药物的选择和感染控制的介入。
목적：비교포만불동간균적분자생물학감정결과화의기감정결과。방법채용법국매리애공사VITEK2 COMPACT전자동미생물분석의대세균진행감정화항균약물민감성분석,감정위포만불동간균복합군적세균,채용취합매련반응( PCR)검측OXA-51기인。결과68주VITEK2 COMPACT감정위포만불동간균복합군적균주중,50주OXA-51기인양성,학정위포만불동간균；18주OXA-51기인음성,위복합군기타세균。포만불동간균( OXA-51양성)여복합군기타세균( OXA-51음성)비교,대림상상용적탄청매희류、안기당감류、불규낙동류、두포균소류등항균약물적민감성강저(P<0．05)。결론포만불동간균복합군기타세균내약솔현저저우포만불동간균,채용검측OXA-51기인방법대포만불동간균복합군진행준학적균충감정유리우정학지도림상항균약물적선택화감염공제적개입。
Objective To Evaluate the value of molecular biology and instrument identification of Acinetobacter bauman-nii strains. Methods Acinetobacter baumannii-A. calcoaceticus complex identification and drug resistance rate was detected using VITEK2 COMPACT of biomerieux. The drug resistance gene OXA-51 was detected by polymerase chain reaction assay. Results Of 68 isolates selected for analysis, 50(73. 53%) were OXA-51-like positive. These isolates were confirmed to be Acinetobacter baumannii. 18 of the 68 isolates were OXA-51-like negative. Comparing with OXA-51-like positive Acinetobacter baumannii, the resistance rates of OXA-51-like negative isolates to carbapenems, aminoglycosides, fluoroquinolones, and cephems were decreased ( P<0. 05 ) . Conclusion Acinetobacter baumannii was more resistant to antibiotics than other Acinetobacter baumannii-A. cal-coaceticus complex isolates. It is important for us to identify Acinetobacter baumannii exactly by detecting OXA-51-like gene in order to guide the clinical selection of antibiotics and nosocomial infection control.