CAJ | 학술논문

建立了高效液相色谱串联质谱法同时测定白酒中6种人工合成甜味剂（安赛蜜、糖精钠、甜蜜素、阿斯巴甜、纽甜及三氯蔗糖）的分析方法。样品直接稀释，过0.45滋m滤膜上机分析。采用C18柱分离，以0.1%甲酸水溶液-甲醇为流动相，梯度洗脱，以保留时间和质荷比对分离出的组分进行定性，用峰面积进行定量。结果表明，6种甜味剂的质量浓度与其峰面积在一定范围内呈良好线性关系，10～200 ng/mL范围内相关系数为0.9915～0.9943，20～500 ng/mL范围内相关系数为0.9961～0.9983，检出限均为10滋g/kg，相对标准偏差2.1%～7.9%。
건립료고효액상색보천련질보법동시측정백주중6충인공합성첨미제（안새밀、당정납、첨밀소、아사파첨、뉴첨급삼록자당）적분석방법。양품직접희석，과0.45자m려막상궤분석。채용C18주분리，이0.1%갑산수용액-갑순위류동상，제도세탈，이보류시간화질하비대분리출적조분진행정성，용봉면적진행정량。결과표명，6충첨미제적질량농도여기봉면적재일정범위내정량호선성관계，10～200 ng/mL범위내상관계수위0.9915～0.9943，20～500 ng/mL범위내상관계수위0.9961～0.9983，검출한균위10자g/kg，상대표준편차2.1%～7.9%。
In this study, we developed a method to simultaneously detect six artificial sweeteners(acesulfame K, saccharin sodium, sodium cycla-mate, aspartame, neotame and sucralose) in Jiangxiang Baijiu (liquor) by HPLC-MS/MS. Samples were diluted directly, and went through 0.45μm filter membrane. Then samples were separated by C18 column, using 0.1%formic acid and methanol as mobile phase, with gradient elu-tion. Qualitative analysis of the separated composition was carried out based on retention time and mass-charge ratio, and quantitative analysis was based on peak area. The results showed that, there was a linear relationship between the mass concentration and the peak area of the six sweeteners:within the range of 10~200 ng/mL, the correlation coefficient was 0.9915~0.9943, and within the range of 20~500 ng/mL, the cor-relation coefficient was 0.9961~0.9983. The detection limit was 10 μg/g, and the relative standard deviation was 2.1%~7.9%. (Trans. by HUANG Xiaoli).