CAJ | 학술논문

利用高氏平板从酱香型白酒生产环境土壤中筛选分离得到1株放线菌NS01，利用形态学、16S rRNA序列同源性鉴定目的菌株为链霉菌属（Streptomyces sp.）。分别采用液态与固态培养基培养目的菌种，通过顶空固相微萃取技术进行萃取，再利用气相色谱质谱联用仪分析代谢产物，结果发现该菌代谢大量的萜烯类物质。代谢产物中的倍半萜烯类物质：2-甲基异莰醇、土臭素具有土臭味和霉味，该类物质可能与酱香型白酒酿造过程中出现霉味有关。
이용고씨평판종장향형백주생산배경토양중사선분리득도1주방선균NS01，이용형태학、16S rRNA서렬동원성감정목적균주위련매균속（Streptomyces sp.）。분별채용액태여고태배양기배양목적균충，통과정공고상미췌취기술진행췌취，재이용기상색보질보련용의분석대사산물，결과발현해균대사대량적첩희류물질。대사산물중적배반첩희류물질：2-갑기이감순、토취소구유토취미화매미，해류물질가능여장향형백주양조과정중출현매미유관。
An actinomycetes strain NS01 was isolated from the soil in the production environment of Maotai by use of Gause's culture plate. And it was identified as Streptomyces sp. by 16S rRNA gene sequence analysis and morphological analysis. After the culture of strain NS01 by liquid culture medium and by solid culture medium respectively, its metabolites were extracted by HS-SPME and then analyzed by GC-MS. It was found that its metabolites contained a large number of terpenes. 2-methylisoborneol and geosmin in the sesquiterpenes in the metabolites might be relat ed to the musty-odor of base liquor.