CAJ | 학술논문

目的：研究人卵巢颗粒细胞胰岛素受体（insulin receptor，INSR）和胰岛素样生长因子-1受体（insulin-like growth factor 1 receptor，IGF-1R）的表达及相关性。方法接受体外受精-胚胎移植（ in vitro fertilization-embryo transfer，IVF-ET）不孕症患者84例，按照妊娠结果分为妊娠组和未妊娠组，每组42例。应用实时荧光定量PCR（ real-time PCR）法测定取排卵日成熟卵泡壁颗粒细胞INSR，IGF-1R mRNA的表达；并对INSR，IGF-1R mRNA之间及与血清LH、E2、P水平相关性分析。结果妊娠组血清雌二醇水平高于未妊娠组（ P ＜0照．05）。妊娠组优质胚胎数高于未妊娠组（ P ＜0．05）。其余两组比较，差异无统计学意义（ P ＞0．05）。妊娠组INSR、IGF-1R、表达高于未妊娠组（ P ＜0．05）。INSR与IGF-1R呈正相关（ r ＝0．712， P ＜0．01）。结论颗粒细胞INSR、IGF-1R可能参与卵母细胞质量的调节。
목적：연구인란소과립세포이도소수체（insulin receptor，INSR）화이도소양생장인자-1수체（insulin-like growth factor 1 receptor，IGF-1R）적표체급상관성。방법접수체외수정-배태이식（ in vitro fertilization-embryo transfer，IVF-ET）불잉증환자84례，안조임신결과분위임신조화미임신조，매조42례。응용실시형광정량PCR（ real-time PCR）법측정취배란일성숙란포벽과립세포INSR，IGF-1R mRNA적표체；병대INSR，IGF-1R mRNA지간급여혈청LH、E2、P수평상관성분석。결과임신조혈청자이순수평고우미임신조（ P ＜0조．05）。임신조우질배태수고우미임신조（ P ＜0．05）。기여량조비교，차이무통계학의의（ P ＞0．05）。임신조INSR、IGF-1R、표체고우미임신조（ P ＜0．05）。INSR여IGF-1R정정상관（ r ＝0．712， P ＜0．01）。결론과립세포INSR、IGF-1R가능삼여란모세포질량적조절。
Objective To investigate the expression of insulin receptor in human ovary granulosa cells ( INSR) and insulin-like growth factor 1 receptor (IGF-1R) and their correlation. Methods Eighty-four patients with infertility received in vitro fertilization and embryo transplantation ( IVF-ET ) , the patients were divided into pregnancy groups ( n =42 ) and non-pregnancy group ( n =42).The expressions levels of INSR and IGF-1R mRNA in mature follicle wall granulosa cells were detected by Real-time PCR.The correlation between INSR , IGF-1R and serum sex hormones was analyzed .Results The high-quality embryo numbers in pregnancy groups were significantly higher than those in non -pregnancy group ( P <0 .05 ) . However there were no significant differences in the other sex hormones levels including LH ,E2,P between the two groups ( P>0.05).The levels of INSR and IGF-1R in pregnancy groups were significantly higher than those in non-pregnancy group ( P<0.05).Moreover the expression of INSR was positively correlated to that of IGF-1R ( r =0.712, P <0.01).Conclusion INSR in human ovary granulosa cells and IGF-1R may be involved in the regulation of oocyte quality .