CAJ | 학술논문

目的：探讨MTHFD1基因多态性与大肠癌遗传易感性的相关性。方法对96例大肠癌患者及96例健康体检者采取问卷调查表的形式,选用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测的方法。结果大肠癌患者中MTHFD1基因多态性G1958A位点rs2236225等位基因的频率与健康对照组比较有显著性差异(P<0.05)；rs2236225基因型者大肠癌的发病风险增加1.6倍(OR=1.603,95%CI=0.331~2.534,P<0.05)。结论MTHFD1基因多态性密切相关于大肠癌的易感性,MTHFD1多态性G1958A位点rs2236225基因改变是大肠癌的主要发病原因。
목적：탐토MTHFD1기인다태성여대장암유전역감성적상관성。방법대96례대장암환자급96례건강체검자채취문권조사표적형식,선용취합매련반응-한제성편단장도다태성(PCR-RFLP)검측적방법。결과대장암환자중MTHFD1기인다태성G1958A위점rs2236225등위기인적빈솔여건강대조조비교유현저성차이(P<0.05)；rs2236225기인형자대장암적발병풍험증가1.6배(OR=1.603,95%CI=0.331~2.534,P<0.05)。결론MTHFD1기인다태성밀절상관우대장암적역감성,MTHFD1다태성G1958A위점rs2236225기인개변시대장암적주요발병원인。
Objective To explore the correlation of MTHFD1 gene polymorphism with genetic susceptibility to colorec-tal cancer. Methods Ninety-six cases of colorectal cancer patients and 96 cases of healthy controls were investigated by questionnaire, the polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) detection method was applied. Results Compared with normal control group, MTHFD1 G1958A polymorphism rs2236225 locus allele frequencies of patients with colorectal cancer had significant difference(P<0.05);rs2236225 genotype risk of col-orectal cancer increased 1.6 times(OR=1.603, 95%CI=0.331~2.534, P<0.05). Conclusion MTHFD1 gene polymorphism is closely related to the susceptibility of colorectal cancer, the main course of colorectal cancer is the rs2236225 gen changes in G1958A site of MTHFD1.