CAJ | 학술논문

目的：研究胃动素对大鼠下丘脑PVN神经元细胞电压依赖性钾电流的影响。方法：采用急性分离新生Wistar大鼠下丘脑细胞和全细胞膜片钳技术的方法，观察胃动素对大鼠下丘脑PVN神经元细胞电压依赖性钾电流的影响。结果：胃动素可抑制大鼠下丘脑PVN神经元电压依赖性钾电流，使电流由（5.406±0.86）nA降至（3.621±0.78）nA，差异有统计学意义（P<0.05）。步进电刺激下丘脑神经元，随着刺激电压强度的增强，钾外向电流增加，使电压-电流曲线（Ⅰ~Ⅴ曲线）右移，但不改变Ⅰ~Ⅴ曲线的形态。结论：胃动素可通过抑制大鼠下丘脑PVN神经元细胞电压依赖性钾电流，发挥其增强胃肠运动的作用。
목적：연구위동소대대서하구뇌PVN신경원세포전압의뢰성갑전류적영향。방법：채용급성분리신생Wistar대서하구뇌세포화전세포막편겸기술적방법，관찰위동소대대서하구뇌PVN신경원세포전압의뢰성갑전류적영향。결과：위동소가억제대서하구뇌PVN신경원전압의뢰성갑전류，사전류유（5.406±0.86）nA강지（3.621±0.78）nA，차이유통계학의의（P<0.05）。보진전자격하구뇌신경원，수착자격전압강도적증강，갑외향전류증가，사전압-전류곡선（Ⅰ~Ⅴ곡선）우이，단불개변Ⅰ~Ⅴ곡선적형태。결론：위동소가통과억제대서하구뇌PVN신경원세포전압의뢰성갑전류，발휘기증강위장운동적작용。
Objective:To study the influence of motilin on the voltage-dependent K+ current of hypothalamic PVN neurons.Method:Patch clamp experiment:rat hypothalamic PVN neurons cells were acute dissociated and observed the influence of motilin on voltage-dependent K+ current of hypothalamic PVN neurons using whole-cell patch-clamp techniques.Result:Motilin could inhibit the voltage-dependent K+ current of rat hypothalamic PVN neurons.The current was decreased from (5.406±0.86)nA to (3.621±0.78)nA,the difference was statistically significant(P<0.05). Step electric stimulation of hypothalamic neurons,with stimulus voltage strength increased,outward potassium current increased,and the Ⅰ-Ⅴ curve shifted right,but the form of the Ⅰ-Ⅴ curve was not changed.Conclusion:Motilin can enhance the motility of gastrointestinal through inhibiting the voltage-dependent K+ current of hypothalamic PVN neurons.