中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
25期
1-3
,共3页
周辛欣%张艳%季峰%顾梦立%钟卫祥
週辛訢%張豔%季峰%顧夢立%鐘衛祥
주신흔%장염%계봉%고몽립%종위상
胃肠道间质瘤%转录调控因子%肿瘤发生
胃腸道間質瘤%轉錄調控因子%腫瘤髮生
위장도간질류%전록조공인자%종류발생
Gastrointestinal stromal tumor%Transcription regulatory factors%Tumorigenesis
目的:探讨ETV1在胃肠道间质瘤组织中的表达及临床意义。方法:应用免疫组化、实时荧光定量PCR、Western-blot检测ETV1在135例不同胃肠道间质瘤患者的肿瘤组织及瘤旁组织中的表达。结果:免疫组化结果显示,ETV1在胃肠道间质瘤中阳性表达率为68.1%,显著高于瘤旁正常组织的5.2%(P<0.05)。荧光定量PCR方法显示,间质瘤组织中ETV1的相对表达Ct值(3.30±2.37)低于瘤旁正常组织的(6.19±2.86),肿瘤组织中ETV1的表达是正常组织的7.41倍,差异有统计学意义(P<0.001)。Western-blot法检测显示, ETV1蛋白在胃肠道间质瘤中的表达水平(1.24±0.67)也较瘤旁正常组织高(0.88±0.28)(P=0.0369);Pearson相关分析显示,ETV1mRNA和蛋白水平呈正相关(r=0.526,P=0.017)。结论:ETV1在胃肠道间质瘤中特异性高表达,可能与胃肠道间质瘤的发生发展有关,有望成为新的肿瘤标志物,为临床治疗提供新靶点。
目的:探討ETV1在胃腸道間質瘤組織中的錶達及臨床意義。方法:應用免疫組化、實時熒光定量PCR、Western-blot檢測ETV1在135例不同胃腸道間質瘤患者的腫瘤組織及瘤徬組織中的錶達。結果:免疫組化結果顯示,ETV1在胃腸道間質瘤中暘性錶達率為68.1%,顯著高于瘤徬正常組織的5.2%(P<0.05)。熒光定量PCR方法顯示,間質瘤組織中ETV1的相對錶達Ct值(3.30±2.37)低于瘤徬正常組織的(6.19±2.86),腫瘤組織中ETV1的錶達是正常組織的7.41倍,差異有統計學意義(P<0.001)。Western-blot法檢測顯示, ETV1蛋白在胃腸道間質瘤中的錶達水平(1.24±0.67)也較瘤徬正常組織高(0.88±0.28)(P=0.0369);Pearson相關分析顯示,ETV1mRNA和蛋白水平呈正相關(r=0.526,P=0.017)。結論:ETV1在胃腸道間質瘤中特異性高錶達,可能與胃腸道間質瘤的髮生髮展有關,有望成為新的腫瘤標誌物,為臨床治療提供新靶點。
목적:탐토ETV1재위장도간질류조직중적표체급림상의의。방법:응용면역조화、실시형광정량PCR、Western-blot검측ETV1재135례불동위장도간질류환자적종류조직급류방조직중적표체。결과:면역조화결과현시,ETV1재위장도간질류중양성표체솔위68.1%,현저고우류방정상조직적5.2%(P<0.05)。형광정량PCR방법현시,간질류조직중ETV1적상대표체Ct치(3.30±2.37)저우류방정상조직적(6.19±2.86),종류조직중ETV1적표체시정상조직적7.41배,차이유통계학의의(P<0.001)。Western-blot법검측현시, ETV1단백재위장도간질류중적표체수평(1.24±0.67)야교류방정상조직고(0.88±0.28)(P=0.0369);Pearson상관분석현시,ETV1mRNA화단백수평정정상관(r=0.526,P=0.017)。결론:ETV1재위장도간질류중특이성고표체,가능여위장도간질류적발생발전유관,유망성위신적종류표지물,위림상치료제공신파점。
Objective:To investigate the expression of ETV1 in gastrointestinal stromal tumors and its clinical significance.Method:Surgical gastrointestinal stromal tumor tissues and adjacent normal mucosa were obtained from 135 patients with gastrointestinal stromal tumor.Immunohistochemistry was conducted to detect the distribution of ETV1 protein,while the quantitative real-time reverse transcriptase polymerase chain reaction and Western blot method were used to detect the expressions of ETV1 mRNA and protein.Result:Immunohistochemical analysis showed the positive rate of ETV1 expression was 68.1% in gastrointestinal stromal tumor tissues,it was significantly higher than 5.2% in the adjacent normal samples(P<0.05).Fluorescence quantitative polymerase chain reaction (PCR) showed that relative expression of Ct value was (3.30±2.37)in gastrointestinal stromal tumor tissues,it was significantly lower than (6.19±2.86) in adjacent normal samples,expression of ETV1 in tumor tissue was 7.41 times of normal tissue,the difference was statistically significant(P<0.001).Western blot analysis revealed that the expression of ETV1 protein was (1.24±0.67) in gastrointestinal stromal tumor tissues,it was significantly higher than (0.88±0.28) in normal colorectal tissues (P=0.0369).Pearson correlation analysis showed that the mRNA level was in positive correlation with the protein level(r=0.526,P=0.017).Conclusion:The specific expression of the ETV1 is in a correlation with the pathogenesis of gastrointestinal stromal tumor.ETV1 is expected to be a new tumor marker and the new target for clinical therapy.