中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
25期
1-3
,共3页
周辛欣%张艳%季峰%顾梦立%钟卫祥
週辛訢%張豔%季峰%顧夢立%鐘衛祥
주신흔%장염%계봉%고몽립%종위상
胃肠道间质瘤%转录调控因子%肿瘤发生
胃腸道間質瘤%轉錄調控因子%腫瘤髮生
위장도간질류%전록조공인자%종류발생
Gastrointestinal stromal tumor%Transcription regulatory factors%Tumorigenesis
目的：探讨ETV1在胃肠道间质瘤组织中的表达及临床意义。方法：应用免疫组化、实时荧光定量PCR、Western-blot检测ETV1在135例不同胃肠道间质瘤患者的肿瘤组织及瘤旁组织中的表达。结果：免疫组化结果显示，ETV1在胃肠道间质瘤中阳性表达率为68.1%，显著高于瘤旁正常组织的5.2%（P<0.05）。荧光定量PCR方法显示，间质瘤组织中ETV1的相对表达Ct值（3.30±2.37）低于瘤旁正常组织的（6.19±2.86），肿瘤组织中ETV1的表达是正常组织的7.41倍，差异有统计学意义（P<0.001）。Western-blot法检测显示， ETV1蛋白在胃肠道间质瘤中的表达水平（1.24±0.67）也较瘤旁正常组织高（0.88±0.28）（P=0.0369）；Pearson相关分析显示，ETV1mRNA和蛋白水平呈正相关（r=0.526，P=0.017）。结论：ETV1在胃肠道间质瘤中特异性高表达，可能与胃肠道间质瘤的发生发展有关，有望成为新的肿瘤标志物，为临床治疗提供新靶点。
目的：探討ETV1在胃腸道間質瘤組織中的錶達及臨床意義。方法：應用免疫組化、實時熒光定量PCR、Western-blot檢測ETV1在135例不同胃腸道間質瘤患者的腫瘤組織及瘤徬組織中的錶達。結果：免疫組化結果顯示，ETV1在胃腸道間質瘤中暘性錶達率為68.1%，顯著高于瘤徬正常組織的5.2%（P<0.05）。熒光定量PCR方法顯示，間質瘤組織中ETV1的相對錶達Ct值（3.30±2.37）低于瘤徬正常組織的（6.19±2.86），腫瘤組織中ETV1的錶達是正常組織的7.41倍，差異有統計學意義（P<0.001）。Western-blot法檢測顯示， ETV1蛋白在胃腸道間質瘤中的錶達水平（1.24±0.67）也較瘤徬正常組織高（0.88±0.28）（P=0.0369）；Pearson相關分析顯示，ETV1mRNA和蛋白水平呈正相關（r=0.526，P=0.017）。結論：ETV1在胃腸道間質瘤中特異性高錶達，可能與胃腸道間質瘤的髮生髮展有關，有望成為新的腫瘤標誌物，為臨床治療提供新靶點。
목적：탐토ETV1재위장도간질류조직중적표체급림상의의。방법：응용면역조화、실시형광정량PCR、Western-blot검측ETV1재135례불동위장도간질류환자적종류조직급류방조직중적표체。결과：면역조화결과현시，ETV1재위장도간질류중양성표체솔위68.1%，현저고우류방정상조직적5.2%（P<0.05）。형광정량PCR방법현시，간질류조직중ETV1적상대표체Ct치（3.30±2.37）저우류방정상조직적（6.19±2.86），종류조직중ETV1적표체시정상조직적7.41배，차이유통계학의의（P<0.001）。Western-blot법검측현시， ETV1단백재위장도간질류중적표체수평（1.24±0.67）야교류방정상조직고（0.88±0.28）（P=0.0369）；Pearson상관분석현시，ETV1mRNA화단백수평정정상관（r=0.526，P=0.017）。결론：ETV1재위장도간질류중특이성고표체，가능여위장도간질류적발생발전유관，유망성위신적종류표지물，위림상치료제공신파점。
Objective:To investigate the expression of ETV1 in gastrointestinal stromal tumors and its clinical significance.Method:Surgical gastrointestinal stromal tumor tissues and adjacent normal mucosa were obtained from 135 patients with gastrointestinal stromal tumor.Immunohistochemistry was conducted to detect the distribution of ETV1 protein,while the quantitative real-time reverse transcriptase polymerase chain reaction and Western blot method were used to detect the expressions of ETV1 mRNA and protein.Result:Immunohistochemical analysis showed the positive rate of ETV1 expression was 68.1% in gastrointestinal stromal tumor tissues,it was significantly higher than 5.2% in the adjacent normal samples(P<0.05).Fluorescence quantitative polymerase chain reaction (PCR) showed that relative expression of Ct value was (3.30±2.37)in gastrointestinal stromal tumor tissues,it was significantly lower than (6.19±2.86) in adjacent normal samples,expression of ETV1 in tumor tissue was 7.41 times of normal tissue,the difference was statistically significant(P<0.001).Western blot analysis revealed that the expression of ETV1 protein was (1.24±0.67) in gastrointestinal stromal tumor tissues,it was significantly higher than (0.88±0.28) in normal colorectal tissues (P=0.0369).Pearson correlation analysis showed that the mRNA level was in positive correlation with the protein level(r=0.526,P=0.017).Conclusion:The specific expression of the ETV1 is in a correlation with the pathogenesis of gastrointestinal stromal tumor.ETV1 is expected to be a new tumor marker and the new target for clinical therapy.
