CAJ | 학술논문

目的：观察肝性脑病大鼠脑海马 CA1区神经元形态的变化及一氧化氮合酶（NOS）的表达情况，探讨海马 CA1区神经元的形态学改变及一氧化氮（ NO）在肝性脑病发病机制中的作用。方法雄性大鼠50只，实验开始前所有动物进行莫里斯水迷宫测试，之后将动物分为正常对照组和实验模型组。9周后建立 CCl4肝性脑病模型，分别取2组大鼠海马组织进行尼氏染色及 NADPH - d 染色。结果尼氏染色显示：实验组大鼠海马神经元数目减少、染色较浅，胞浆内尼氏体减少或消失。NADPH - d 染色显示：实验组可见粗大轴突着色，树突联系广泛；对照组则少有粗大轴突着色，树突间联系不如实验组广泛。实验组 NOS 阳性神经元染色较对照组深，为紫蓝或深蓝色（强阳性及阳性），且阳性神经元数目较多；而对照组染色浅淡，呈浅蓝或与背景同色，为弱阳性。结论肝性脑病时海马受到损伤，并且 NO 可能介导了神经元的损伤并参与了肝硬化和肝性脑病的发病。
목적：관찰간성뇌병대서뇌해마 CA1구신경원형태적변화급일양화담합매（NOS）적표체정황，탐토해마 CA1구신경원적형태학개변급일양화담（ NO）재간성뇌병발병궤제중적작용。방법웅성대서50지，실험개시전소유동물진행막리사수미궁측시，지후장동물분위정상대조조화실험모형조。9주후건립 CCl4간성뇌병모형，분별취2조대서해마조직진행니씨염색급 NADPH - d 염색。결과니씨염색현시：실험조대서해마신경원수목감소、염색교천，포장내니씨체감소혹소실。NADPH - d 염색현시：실험조가견조대축돌착색，수돌련계엄범；대조조칙소유조대축돌착색，수돌간련계불여실험조엄범。실험조 NOS 양성신경원염색교대조조심，위자람혹심람색（강양성급양성），차양성신경원수목교다；이대조조염색천담，정천람혹여배경동색，위약양성。결론간성뇌병시해마수도손상，병차 NO 가능개도료신경원적손상병삼여료간경화화간성뇌병적발병。
Objective It is to observe the morphological changes of neurons and the NOS expression in CA1 hippocampus of rats,and investigate the morphological changes of hippocampal neurons and the role of nitric oxide(NO)in the pathogene-sis of hepatic encephalopathy. Methods 50 cases of male Wistar rats were divided into control group and experimental group, before the experiment all the rats were tested by Morris water maze test,after 9 weeks CCl4 model of hepatic encephalopathy was established,the hippocampus of each groups were take out for Nissl staining and dyeing of NADPH - d. Results Nissl staining results:in experimental group,the number of neurons in hippocampus was less than control group and dyed lighter, Nissl bodies was also reduced. NADPH - d staining results:in experimental group,axon was dyed deeper,widespread den-drites connections were observed. In control group there is few positive axon and less connections between dendrites. NADPH - d staining results:the color in experimental group was blue or deep blue showed positive or strong positive,while in control group showed weak positive. Conclusion Hepatic encephalopathy can lead to hippocampus injury,NO may mediated the process.