现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
27期
2980-2982
,共3页
慢性肺栓塞%HMGB-1%IL-1β%D-二聚体
慢性肺栓塞%HMGB-1%IL-1β%D-二聚體
만성폐전새%HMGB-1%IL-1β%D-이취체
chronic pulmonary embolism%HMGB - 1%IL - 1β%D - Dimer
目的：观测慢性肺栓塞（ CPE）患者血清中 HMGB -1的表达水平及其与 IL -1β、TNF -α、D -二聚体表达水平的相关性。方法 CPE 组选取 CPE 患者38例，对照组选取同期健康体检者42例。采用酶联免疫吸附（ ELISA）方法检测2组血清中 HMGB -1、IL -1β、TNF -α蛋白的表达水平，采用免疫比浊法测定血清中 D -二聚体表达水平，采用荧光定量 PCR 方法检测外周血单核细胞（PBMC）中 HMGB -1 mRNA 的表达水平。结果 CPE 组血清中 HMGB -1、IL -1β、TNF -α、D -二聚体的表达均明显高于对照组（P 均﹤0.05），且血清中 HMGB -1蛋白水平与 IL -1β、TNF -α、D -二聚体水平均呈正相关（ P 均﹤0.05)。CPE 组外周血单核细胞 HMGB -1 mRNA 表达量明显高于健康对照组( P ﹤0.05)。结论 HMGB
目的：觀測慢性肺栓塞（ CPE）患者血清中 HMGB -1的錶達水平及其與 IL -1β、TNF -α、D -二聚體錶達水平的相關性。方法 CPE 組選取 CPE 患者38例，對照組選取同期健康體檢者42例。採用酶聯免疫吸附（ ELISA）方法檢測2組血清中 HMGB -1、IL -1β、TNF -α蛋白的錶達水平，採用免疫比濁法測定血清中 D -二聚體錶達水平，採用熒光定量 PCR 方法檢測外週血單覈細胞（PBMC）中 HMGB -1 mRNA 的錶達水平。結果 CPE 組血清中 HMGB -1、IL -1β、TNF -α、D -二聚體的錶達均明顯高于對照組（P 均﹤0.05），且血清中 HMGB -1蛋白水平與 IL -1β、TNF -α、D -二聚體水平均呈正相關（ P 均﹤0.05)。CPE 組外週血單覈細胞 HMGB -1 mRNA 錶達量明顯高于健康對照組( P ﹤0.05)。結論 HMGB
목적：관측만성폐전새（ CPE）환자혈청중 HMGB -1적표체수평급기여 IL -1β、TNF -α、D -이취체표체수평적상관성。방법 CPE 조선취 CPE 환자38례，대조조선취동기건강체검자42례。채용매련면역흡부（ ELISA）방법검측2조혈청중 HMGB -1、IL -1β、TNF -α단백적표체수평，채용면역비탁법측정혈청중 D -이취체표체수평，채용형광정량 PCR 방법검측외주혈단핵세포（PBMC）중 HMGB -1 mRNA 적표체수평。결과 CPE 조혈청중 HMGB -1、IL -1β、TNF -α、D -이취체적표체균명현고우대조조（P 균﹤0.05），차혈청중 HMGB -1단백수평여 IL -1β、TNF -α、D -이취체수평균정정상관（ P 균﹤0.05)。CPE 조외주혈단핵세포 HMGB -1 mRNA 표체량명현고우건강대조조( P ﹤0.05)。결론 HMGB
Objective It is to observe the expression of HMGB - 1 in patients with chronic pulmonary embolism( CPE) and its correlation with the expression of IL - 1β,TNF - α and D - dimer. Methods 38 patients of CPE were selected into CPE group and 42 healthy subjects in the same period in were selected into control group. The expressions of serum HMGB -1,IL - 1β,TNF - αwere detected by ELISA method. D - Dimer was detected by immunoturbidimetry. HMGB - 1 mRNA ex-pression was detected by Real-time PCR in PBMC. Results The expression levels of HMGB - 1,IL - 1β,TNF - α,D - Di-mer in CPE group were significantly higher than those in control group(P ﹤ 0. 05)and the level of HMGB - 1 protein in serum of CPE patients was positively correlated with IL - 1β,TNF - α,D - Dimer(P ﹤ 0. 05). The expression of HMGB - 1 mRNA in CPE group was significantly higher than those in control group( P ﹤ 0. 05). Conclusion The increase in HMGB - 1 can serve as a potential diagnosis index for CPE and the mechanism may be of the HMGB - 1 mRNA transcription enhancement stimulated by other factors in CPE patients PBMC.
