中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
9期
769-772
,共4页
刘高华%陈冠婕%陈怡环%杨帆%张瑞国%彭正午%谭庆荣
劉高華%陳冠婕%陳怡環%楊帆%張瑞國%彭正午%譚慶榮
류고화%진관첩%진이배%양범%장서국%팽정오%담경영
重复经颅磁刺激%脑源性神经营养因子%核因子κB%白介素-1β%大鼠
重複經顱磁刺激%腦源性神經營養因子%覈因子κB%白介素-1β%大鼠
중복경로자자격%뇌원성신경영양인자%핵인자κB%백개소-1β%대서
Repetitive transcranial magnetic stimulation%BDNF%NF-κB%IL-1β%Rats
目的 观察重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)对慢性不可预见性温和应激(chronic unpredictable mild stress,CUMS)抑郁模型大鼠行为的改善作用及对脑源性神经营养因子(brain derived beurotrophic factor,BDNF),白介素-1β (IL-1β),核因子κB(nuclear factor-κB,NF-κB)表达的影响.方法 32只成年雄性SD大鼠随机分为Control组、Control+ rTMS组、CUMS组、CUMS+ rTMS组.CUMS+rTMS组接受为期21d的造模处理,随后接受连续7d的rTMS干预；CUMS组大鼠也进行21d的造模处理,但是接受连续7d的rTMS假刺激；Control和Control+ rTMS组正常饲养21d后,接受相应的rTMS假刺激或真刺激,为期7d.各组在处理结束后采用糖水偏好实验、强迫游泳实验、旷场实验检测大鼠抑郁样行为表现,并且在行为学实验完成后处死大鼠,以蛋白质印迹法(Western blot)检测海马BDNF、NF-κB的表达水平,以酶联免疫吸附法(ELISA)检测海马IL-1β的含量.结果 1.rTMS对CUMS大鼠抑郁样行为的影响:糖水偏好实验中,CUMS组大鼠的糖水偏好系数(0.67±0.06)较Control组(0.91±0.04)明显减小,而CUMS+ rTMS组大鼠的糖水偏好系数(0.83±0.08)增加；强迫游泳实验中,CUMS组大鼠的不动时间[(26.88±11.33)s]较Control组[(15.22 ±6.75)s]延长,而CUMS+ rTMS组大鼠不动时间[(18.41 ±6.95)s]缩短；旷场实验中,CUMS组大鼠的自发活动总距离[(849.17±769.01)cm]与进入旷场中心区次数[(7.42±5.68)次]均显著低于Control组[(6224.81±1403.20)cm,(22.86 ±3.72)次],而CUMS+ rTMS组大鼠的自发活动总距离[(4105.57±1516.92) cm]与进入旷场中心区次数[(21.25±3.45)次]增多,差异均有统计学意义(均P＜0.05).Control组与Control+ rTMS组的上述行为学指标之间均差异无统计学意义.2.rTMS对CUMS大鼠海马BDNF、NF-κB和IL-1β水平的影响:与Control组相比,CUMS组海马BDNF表达降低,而NF-κB的表达及IL-1β水平明显增高(均P＜0.05),而与CUMS组大鼠相比,CUMS+ rTMS组BDNF表达增高、NF-κB表达水平及IL-1β水平均降低(均P＜0.05).Control组与Control+ rTMS组NF-κB、BDNF的表达情况及IL-1β的水平均差异均无统计学意义.结论 rTMS干预能改善CUMS模型大鼠抑郁样行为,并且这种作用可能是通过上调海马BDNF的表达,降低NF-κB以及IL-1β水平实现的.
目的 觀察重複經顱磁刺激(repetitive transcranial magnetic stimulation,rTMS)對慢性不可預見性溫和應激(chronic unpredictable mild stress,CUMS)抑鬱模型大鼠行為的改善作用及對腦源性神經營養因子(brain derived beurotrophic factor,BDNF),白介素-1β (IL-1β),覈因子κB(nuclear factor-κB,NF-κB)錶達的影響.方法 32隻成年雄性SD大鼠隨機分為Control組、Control+ rTMS組、CUMS組、CUMS+ rTMS組.CUMS+rTMS組接受為期21d的造模處理,隨後接受連續7d的rTMS榦預；CUMS組大鼠也進行21d的造模處理,但是接受連續7d的rTMS假刺激；Control和Control+ rTMS組正常飼養21d後,接受相應的rTMS假刺激或真刺激,為期7d.各組在處理結束後採用糖水偏好實驗、彊迫遊泳實驗、曠場實驗檢測大鼠抑鬱樣行為錶現,併且在行為學實驗完成後處死大鼠,以蛋白質印跡法(Western blot)檢測海馬BDNF、NF-κB的錶達水平,以酶聯免疫吸附法(ELISA)檢測海馬IL-1β的含量.結果 1.rTMS對CUMS大鼠抑鬱樣行為的影響:糖水偏好實驗中,CUMS組大鼠的糖水偏好繫數(0.67±0.06)較Control組(0.91±0.04)明顯減小,而CUMS+ rTMS組大鼠的糖水偏好繫數(0.83±0.08)增加；彊迫遊泳實驗中,CUMS組大鼠的不動時間[(26.88±11.33)s]較Control組[(15.22 ±6.75)s]延長,而CUMS+ rTMS組大鼠不動時間[(18.41 ±6.95)s]縮短；曠場實驗中,CUMS組大鼠的自髮活動總距離[(849.17±769.01)cm]與進入曠場中心區次數[(7.42±5.68)次]均顯著低于Control組[(6224.81±1403.20)cm,(22.86 ±3.72)次],而CUMS+ rTMS組大鼠的自髮活動總距離[(4105.57±1516.92) cm]與進入曠場中心區次數[(21.25±3.45)次]增多,差異均有統計學意義(均P＜0.05).Control組與Control+ rTMS組的上述行為學指標之間均差異無統計學意義.2.rTMS對CUMS大鼠海馬BDNF、NF-κB和IL-1β水平的影響:與Control組相比,CUMS組海馬BDNF錶達降低,而NF-κB的錶達及IL-1β水平明顯增高(均P＜0.05),而與CUMS組大鼠相比,CUMS+ rTMS組BDNF錶達增高、NF-κB錶達水平及IL-1β水平均降低(均P＜0.05).Control組與Control+ rTMS組NF-κB、BDNF的錶達情況及IL-1β的水平均差異均無統計學意義.結論 rTMS榦預能改善CUMS模型大鼠抑鬱樣行為,併且這種作用可能是通過上調海馬BDNF的錶達,降低NF-κB以及IL-1β水平實現的.
목적 관찰중복경로자자격(repetitive transcranial magnetic stimulation,rTMS)대만성불가예견성온화응격(chronic unpredictable mild stress,CUMS)억욱모형대서행위적개선작용급대뇌원성신경영양인자(brain derived beurotrophic factor,BDNF),백개소-1β (IL-1β),핵인자κB(nuclear factor-κB,NF-κB)표체적영향.방법 32지성년웅성SD대서수궤분위Control조、Control+ rTMS조、CUMS조、CUMS+ rTMS조.CUMS+rTMS조접수위기21d적조모처리,수후접수련속7d적rTMS간예；CUMS조대서야진행21d적조모처리,단시접수련속7d적rTMS가자격；Control화Control+ rTMS조정상사양21d후,접수상응적rTMS가자격혹진자격,위기7d.각조재처리결속후채용당수편호실험、강박유영실험、광장실험검측대서억욱양행위표현,병차재행위학실험완성후처사대서,이단백질인적법(Western blot)검측해마BDNF、NF-κB적표체수평,이매련면역흡부법(ELISA)검측해마IL-1β적함량.결과 1.rTMS대CUMS대서억욱양행위적영향:당수편호실험중,CUMS조대서적당수편호계수(0.67±0.06)교Control조(0.91±0.04)명현감소,이CUMS+ rTMS조대서적당수편호계수(0.83±0.08)증가；강박유영실험중,CUMS조대서적불동시간[(26.88±11.33)s]교Control조[(15.22 ±6.75)s]연장,이CUMS+ rTMS조대서불동시간[(18.41 ±6.95)s]축단；광장실험중,CUMS조대서적자발활동총거리[(849.17±769.01)cm]여진입광장중심구차수[(7.42±5.68)차]균현저저우Control조[(6224.81±1403.20)cm,(22.86 ±3.72)차],이CUMS+ rTMS조대서적자발활동총거리[(4105.57±1516.92) cm]여진입광장중심구차수[(21.25±3.45)차]증다,차이균유통계학의의(균P＜0.05).Control조여Control+ rTMS조적상술행위학지표지간균차이무통계학의의.2.rTMS대CUMS대서해마BDNF、NF-κB화IL-1β수평적영향:여Control조상비,CUMS조해마BDNF표체강저,이NF-κB적표체급IL-1β수평명현증고(균P＜0.05),이여CUMS조대서상비,CUMS+ rTMS조BDNF표체증고、NF-κB표체수평급IL-1β수평균강저(균P＜0.05).Control조여Control+ rTMS조NF-κB、BDNF적표체정황급IL-1β적수평균차이균무통계학의의.결론 rTMS간예능개선CUMS모형대서억욱양행위,병차저충작용가능시통과상조해마BDNF적표체,강저NF-κB이급IL-1β수평실현적.
Objective To investigate the effect of repetitive transcranial magnetic stimulation (rTMS) on the depressive like behaviors and expression of brain derived neurotrophic factor (BDNF),IL-1β and NF-κB of hippocampal in chronic unpredictable mild stress (CUMS) rats.Methods Thirty-two adult male rats were randomly divided into four groups (n =8):Control group,Control + rTMS group,CUMS group and CUMS + rTMS group.The sucrose preference test,forced swim test and open field test were used to evaluate depressive like behaviors for each groups.In addition,the expression of BDNF,NF-κB and IL-1 β in hippocampal were detected by western blot and ELISA after behavioral test,respectively.Results 1.The effects of rTMS on depressive like behaviors of CUMS rats:in the sucrose preference test,the sucrose preference rate of CUMS rats (0.67 ± 0.06) was significantly lower than Control group (0.91 ± 0.04),which was higher in the CUMS + rTMS group (0.83 ±0.08).In the forced swim test,the immobility time of CUMS group ((26.88 ± 11.33) s) was longer than Control group ((15.22 ± 6.75) s) and CUMS + rTMS group ((18.41 ± 6.95) s).In the open field test,both the total distance travelled and number of central area entry times of CUMS group((849.165 ± 769.01) cm,(7.42 ± 5.68))were significantly shorter ((6224.81 ± 1403.2) cm) and smaller (22.86 ± 3.72) than Control group,and those of the CUMS + rTMS were longer ((4105.57 ± 1516.92)cm) and larger (21.25 ± 3.45).All the behavioral results were statistically significant (P＜ 0.05).And of all the aforementioned behavioral parameters,there were no significant differences between Control group and Control + rTMS group(P＞0.05).2.The effects of rTMS on the hippocampal expressions of BDNF,NF-κB and IL-1β in CUMS rats:compared with Control group,the hippocampal expression of BDNF in CUMS rats was significantly decreased,while the expressions of NF-κB and IL-1β in the hippocampus were significantly increased (P＜ 0.05).Compared with CUMS group,the hippocampa expression of BD-NF in the CUMS + rTMS group was increased,and the expressions of NF-κB and IL-1β in the hippocampus was significantly decreased (P ＜ 0.05).The expressions of BDNF,NF-κB and IL-1β had no differences between Control group and Control + rTMS group.Conclusion rTMS increased the expression of BDNF,reduced the production of NF-κB and IL-1β,and alleviated depressive like behaviors in CUMS rats.
