CAJ | 학술논문

目的研究慢性不可预见性温和应激(CUMS)状态下大鼠胃肠动力功能的变化及其可能的机制.方法将大鼠随机分为空白对照组和应激模型组,采用21d慢性不可预见性温和刺激结合孤养方式复制大鼠慢性应激模型,对照观察两组大鼠在体胃肠道墨水推进率以及离体肠管收缩性的差异,并利用免疫组化方法,比较两组大鼠下丘脑室旁核(P/N)催产素(OT)样免疫阳性物质的表达.结果 (1)造模结束,模型组大鼠体重增加量、糖水偏好[分别(69.97 ±9.81)g,(49.05±5.98)g]低于对照组[分别(116.27±13.60)g,(83.51±3.08)g] (P＜0.001),旷场实验中模型组水平得分、垂直得分[分别(24.00 ±13.52)分,(3.90±2.51)分]低于对照组[分别(53.60±27.98)分,(11.50±8.85)分](P＜0.05)；(2)模型组大鼠墨水推进率[(67.33±6.24)％]低于对照组[(76.83±10.00)％](P＜0.05),离体肠管收缩幅度和收缩频率[分别(1.37±0.18)g,(0.58±0.02) S-1]低于对照组[分别(1.88±0.13)g,(0.62±0.04) S-1] (P＜0.05)；(3)模型组大鼠下丘脑室旁核催产素免疫阳性物质大量表达[(59.17±16.08)个],与对照组[(6.07±3.71)个]比较显著增加(P＜0.001).结论慢性应激可造成大鼠胃肠动力功能下降,这些变化可能与室旁核内催产素表达增多有关.
목적 연구만성불가예견성온화응격(CUMS)상태하대서위장동력공능적변화급기가능적궤제.방법 장대서수궤분위공백대조조화응격모형조,채용21d만성불가예견성온화자격결합고양방식복제대서만성응격모형,대조관찰량조대서재체위장도묵수추진솔이급리체장관수축성적차이,병이용면역조화방법,비교량조대서하구뇌실방핵(P/N)최산소(OT)양면역양성물질적표체.결과 (1)조모결속,모형조대서체중증가량、당수편호[분별(69.97 ±9.81)g,(49.05±5.98)g]저우대조조[분별(116.27±13.60)g,(83.51±3.08)g] (P＜0.001),광장실험중모형조수평득분、수직득분[분별(24.00 ±13.52)분,(3.90±2.51)분]저우대조조[분별(53.60±27.98)분,(11.50±8.85)분](P＜0.05)；(2)모형조대서묵수추진솔[(67.33±6.24)％]저우대조조[(76.83±10.00)％](P＜0.05),리체장관수축폭도화수축빈솔[분별(1.37±0.18)g,(0.58±0.02) S-1]저우대조조[분별(1.88±0.13)g,(0.62±0.04) S-1] (P＜0.05)；(3)모형조대서하구뇌실방핵최산소면역양성물질대량표체[(59.17±16.08)개],여대조조[(6.07±3.71)개]비교현저증가(P＜0.001).결론 만성응격가조성대서위장동력공능하강,저사변화가능여실방핵내최산소표체증다유관.
Objective To study the changes of gastrointestinal movement function in rats with chronic unpredictable mild stress(CUMS) and explore the mechanisms underlying it.Methods The rats were divided into stress model group and control group.The stress model rats were induced by 21-day chronic unpredictable mild stress as well as social-isolated fed.The rate of ink propulsion of gastrointestinal tract and the contraction of intestinal canal in rats were observed.Immunohistochemistry was adopted to detect the expression of OT in rats.Results (1) After the models were induced,weight-gain and sucrose preference of model group ((69.97 ± 9.81) g,(49.05± 5.98) g) were lower than those in control group ((116.27 ± 13.60) g,(83.51 ± 3.08) g) (P ＜ 0.001),and both the crossing-score and rearing-score ((24.00 ± 13.52),(3.90 ± 2.51)) were lower than those in control group ((53.60 ± 27.98),(11.50 ± 8.85)) in the open-field test.(2) The rate of ink propulsion of model group ((67.33 ± 6.24) ％) was decreased when compared to the control group ((76.83 ± 10.00) ％) (P ＜ 0.05),and the intestinal canal contraction amplitude and contraction frequency ((1.37 ± 0.18) g,(0.58 ± 0.02) S-1) were lower than those in control group ((1.88 ± 0.13) g),(0.62 ± 0.04) S-1) (P ＜ 0.05).(3) Compared with the control group (6.07 ± 3.71),OT immunoreactive substance was increased in model group (59.17 ± 16.08) of rats (P＜0.001).Conclusion Chronic stress can cause the decrease in gastrointestinal movement function of rats.These changes may be related to the increased expression of OT in paraventricular nucleus.