CAJ | 학술논문

目的观测水通道蛋白(Aquaporins,AQPs)在慢性应激抑郁模型大鼠组织中的表达,探讨抑郁症部分躯体症状的发病机制.方法采用慢性应激抑郁大鼠模型；将20只大鼠随机分为正常对照组和模型对照组,以免疫组化研究AQP4及AQP3、AQP8分别在抑郁模型大鼠海马及胃肠中的表达.结果正常组及模型组大鼠海马中AQP4平均光密度分别为0.28 ±0.02、0.22 ±0.06,2组比较差异具有统计学意义(t值为2.756,P＜0.05)；2组胃及结肠黏膜中AQP3的表达差异无统计学意义(t值为1.814,1.812,P＞0.05).小肠黏膜中AQP3平均光密度值2组分别为0.15 ±0.02、0.17 ±0.02,差异有统计学意义(t值为2.769,P＜0.05).2组AQP8表达的平均光密度值胃黏膜为0.15±0.01、0.19±0.04；小肠黏膜为0.16±0.01、0.21±0.04；结肠黏膜为0.16±0.01、0.22 ±0.04,差异有统计学意义(t值分别为3.139,5.113,4.534,P＜0.05,P＜0.01,P＜0.01).结论抑郁模型大鼠海马中APQ4的表达低于正常组；胃及结肠黏膜上皮中AQP3表达无明显变化,小肠黏膜表达上调；AQP8在胃、小肠及结肠黏膜中的表达均上调.
목적 관측수통도단백(Aquaporins,AQPs)재만성응격억욱모형대서조직중적표체,탐토억욱증부분구체증상적발병궤제.방법 채용만성응격억욱대서모형；장20지대서수궤분위정상대조조화모형대조조,이면역조화연구AQP4급AQP3、AQP8분별재억욱모형대서해마급위장중적표체.결과 정상조급모형조대서해마중AQP4평균광밀도분별위0.28 ±0.02、0.22 ±0.06,2조비교차이구유통계학의의(t치위2.756,P＜0.05)；2조위급결장점막중AQP3적표체차이무통계학의의(t치위1.814,1.812,P＞0.05).소장점막중AQP3평균광밀도치2조분별위0.15 ±0.02、0.17 ±0.02,차이유통계학의의(t치위2.769,P＜0.05).2조AQP8표체적평균광밀도치위점막위0.15±0.01、0.19±0.04；소장점막위0.16±0.01、0.21±0.04；결장점막위0.16±0.01、0.22 ±0.04,차이유통계학의의(t치분별위3.139,5.113,4.534,P＜0.05,P＜0.01,P＜0.01).결론 억욱모형대서해마중APQ4적표체저우정상조；위급결장점막상피중AQP3표체무명현변화,소장점막표체상조；AQP8재위、소장급결장점막중적표체균상조.
Objective To study the mechanisms of depression by exploring expressional differences of AQPs in the tissues of chronic stress depression rats.Methods Depression model was replicated by unpredicted chronic stress.20 rats were randomly separated into normal control group and model control group,AQP4 and AQP3,AQP8 expressions on hippocampus and gastrointestinal mucosa of rat model with depression were detected by immunochemical staining method.Results Means of optical density of AQP4 of normal group and model group hippocampus were 0.28 ± 0.02,0.22 ± 0.06 respectively,and the difference between two groups was significant statistically(t value was 2.756,P＜0.05).The expression of AQP3 on gastric mucosa and colonic mucosa between two groups had no significant statistically(t value were 1.814,1.812,P＞0.05).Two groups'means of optical density of AQP3 on small intestinal mucosa were 0.15 ± 0.02,0.17 ± 0.02,and the difference was significant statistically (t value was 2.769,P＜0.05).Two groups'means of AQP8 optical density in gastric mucosa were 0.15± 0.01,0.19 ± 0.04 ;0.16 ± 0.01 and 0.21 ± 0.04 in small intestinal mucosa;0.16 ± 0.01 and 0.22 ± 0.04 in colonic mucosa,and the differences were significant statistically(t values were 3.139,5.113,4.534,P＜0.05,P＜0.01,P＜0.01).Conclusion The expression of AQP4 on depression model rat hippocampus are lower than those of the normal group ; and the expression of AQP3 on gastric mucosa and colonic mucosa are no change obviously,but it(')s up-regulation on small intestinal mucosa.The expressions of AQP8 on gastric mucosa and small intestinal mucosa and colonic mucosa are up-regulating.