CAJ | 학술논문

研究武汉地区耐链霉素（ SM）结核分枝杆菌的耐药分子机制，进一步研究阐明rrs基因突变与结核分枝杆菌耐链霉素的关系；同时探究武汉地区结核分枝杆菌“北京基因型”菌株的流行趋势，为进一步研究武汉地区耐链霉素菌株rrs基因突变与“北京基因型”的相关性奠定基础。84株结核分枝杆菌中，44株对链霉素耐药，20株耐其它药物，20株对链霉素敏感，采用PCR直接测序法分析链霉素菌株rrs基因突变情况；采用RD105缺失法鉴定84株临床分离菌株中“北京基因型”菌株。结果显示44株SM耐药菌株中，16（36．4％）株检测到rrs基因突变，其中9株1401位点A→G，6株514位点A→C突变，1株1487位点G→A突变；20株耐其它药菌株中1401位点A→G和514位点A→C突变各1株，20株敏感菌株中发现1株1029位点C→T点突变；84株临床分离菌株中有77（91．8％）株“北京基因型”菌株，7株非“北京基因型”菌株。研究表明链霉素耐药菌株rrs基因主要突变位点在514和1401位点，武汉地区“北京基因型”菌株为当地流行的结核分枝杆菌，且SM耐药菌株中突变菌株93．8％（15/16）为“北京基因型”，“北京基因型”菌株研究及其鉴定是有其科学价值的，值得我们重视。
연구무한지구내련매소（ SM）결핵분지간균적내약분자궤제，진일보연구천명rrs기인돌변여결핵분지간균내련매소적관계；동시탐구무한지구결핵분지간균“북경기인형”균주적류행추세，위진일보연구무한지구내련매소균주rrs기인돌변여“북경기인형”적상관성전정기출。84주결핵분지간균중，44주대련매소내약，20주내기타약물，20주대련매소민감，채용PCR직접측서법분석련매소균주rrs기인돌변정황；채용RD105결실법감정84주림상분리균주중“북경기인형”균주。결과현시44주SM내약균주중，16（36．4％）주검측도rrs기인돌변，기중9주1401위점A→G，6주514위점A→C돌변，1주1487위점G→A돌변；20주내기타약균주중1401위점A→G화514위점A→C돌변각1주，20주민감균주중발현1주1029위점C→T점돌변；84주림상분리균주중유77（91．8％）주“북경기인형”균주，7주비“북경기인형”균주。연구표명련매소내약균주rrs기인주요돌변위점재514화1401위점，무한지구“북경기인형”균주위당지류행적결핵분지간균，차SM내약균주중돌변균주93．8％（15/16）위“북경기인형”，“북경기인형”균주연구급기감정시유기과학개치적，치득아문중시。
The research aims to explore the molecular mechanism of drug-resistant mycobacterium tuberculosis re-sistance to SM in Wuhan area , and elucidate the relationship between rrs gene mutation and mycobacterium tuber-culosis resistance to streptomycin;at the same time to reveal the transmission of Beijing genotype strains of myco-bacterium tuberculosis in Wuhan , and lay foundation for futher study in the correlation between rrsgene mutation and Beijing genotype in SM-resistant clinical isolates .84 strains of mycobacterium tuberculosis were obtained , of which44 were resistant to SM ,20 were resistant to other drugs , 20 were SM susceptible .Direct sequencing analy-sis was used to detect rrs genes , RD105 gene deletion was used to identify the Beijing genotype in the 84 clinical i-solates. Among 44 SM-resistant isolates, rrs gene mutation was detected in 16(36.4%) isolates, 9 took mutation A1401G , 6 took mutation A514C;C1029T mutation was found in one of 20 sensitive strains;A1401G and A514C mutation were found in 20 strains resistant to other drugs;among 84 clinical isolates:77(91.7%) Beijing genotype strains ,7 Beijing genotype strains were found .Our study showed that rrsgene mutation in streptomycin resistant strains mainly happened in 514 and 1401 locus.Beijing genotype was epidemic in Wuhan area and accounted for 93.8%(15/16) in the SM-resistant clinical isolates, suggesting that research and identification of Beijing genotype strains has its scientific value .