CAJ | 학술논문

为开发新的天然烟用香料，研究了超临界CO2萃取白胡椒挥发油的工艺，采用GC-MS法分析了白胡椒挥发油的成分，同时建立了挥发油GC-MS指纹图谱，并进行了方法学考察。结果表明：①超临界CO2萃取白胡椒挥发油的最佳工艺条件为萃取温度45℃、萃取压力35 MPa、萃取时间2 h，原料粉碎至粒径0．3 mm；②以GC-MS方法分析白胡椒挥发油共鉴定出66种成分，主要是萜烯类、醇类、酸类物质；③12个主要成分的出峰次序及其相对含量构成了超临界 CO2萃取白胡椒挥发油的特征指纹图谱；④利用所建立的指纹图谱，可以控制超临界CO2萃取白胡椒挥发油的质量及稳定性。
위개발신적천연연용향료，연구료초림계CO2췌취백호초휘발유적공예，채용GC-MS법분석료백호초휘발유적성분，동시건립료휘발유GC-MS지문도보，병진행료방법학고찰。결과표명：①초림계CO2췌취백호초휘발유적최가공예조건위췌취온도45℃、췌취압력35 MPa、췌취시간2 h，원료분쇄지립경0．3 mm；②이GC-MS방법분석백호초휘발유공감정출66충성분，주요시첩희류、순류、산류물질；③12개주요성분적출봉차서급기상대함량구성료초림계 CO2췌취백호초휘발유적특정지문도보；④이용소건립적지문도보，가이공제초림계CO2췌취백호초휘발유적질량급은정성。
In order to develop new natural cigarette flavors,Pipernigrum L.was extracted with supercritical CO2 .The ob-tained oil was analyzed by GC-MS,and its fingerprints was estabilished,the method was investigated by methodological ap-proach as well .The results showed that:①the optimum extraction conditions were extraction temperature 45 ℃ ,extraction pressure 35 MPa,extraction time 2 h,the appropriate particle size 0.3 mm;②66 components in the extract were identified by GC-MS,mainly including terpenes,alcohols,acids and esters;③12 common peaks with their retention time and peak area of the components appeared the chromatographic fingerprints of this oil;④ the fingerprints would be beneficial for the quality and stability control for piper oil obtained by suppercritical CO2 fluid extraction method.