CAJ | 학술논문

从贵州铜仁产发酵食品中分离纯化出70余株乳酸菌，采用Agar-spot-test初筛与排除酸、过氧化氢抑制后复筛出一株能产广谱抑菌作用细菌素的乳酸菌（编号G55），经生理生化及16S rDNA鉴定可知G55为植物乳杆菌。抑菌谱实验表明，G55产生的细菌素能抑制革兰阳性菌及革兰阴性菌的生长；蛋白酶实验表明，G55产生的细菌素对胃蛋白酶、蛋白酶K敏感，对胰蛋白酶、α凝乳蛋白酶部分敏感。
종귀주동인산발효식품중분리순화출70여주유산균，채용Agar-spot-test초사여배제산、과양화경억제후복사출일주능산엄보억균작용세균소적유산균（편호G55），경생리생화급16S rDNA감정가지G55위식물유간균。억균보실험표명，G55산생적세균소능억제혁란양성균급혁란음성균적생장；단백매실험표명，G55산생적세균소대위단백매、단백매K민감，대이단백매、α응유단백매부분민감。
More than 70 strains of lactic acid bacteria are separated and purified from the fermented foods made in Tongren, Guizhou. First, they are preliminarily sifted by means of agar-spot-test and then excluded from the inhibition of the acid and hydrogen peroxide. After a second sift, a strain of lactic acid bacteria named G55 which can produce broad-spectrum antibacterial bacteriocin is chosen from them. After the physiobiochemical experiment and the 16SrDNA identification, it is concluded that G55 is an actobacillus plantarum. According to the antibacterial spectrum experiment, it shows that the bacteriocin produced by G55 can inhibit the growth of both gram-positive bacteria and gram-negative bacteria. Meanwhile, the experiment of protease shows that the bacteriorin is sensitive to pepsin and proteinase K and is partially sensitive to trypsin andαcurd protease.