CAJ | 학술논문

目的：探讨白细胞介素-1β（ interleukin-1β，IL-1β）、肿瘤坏死因子-α（ tumor necrosis factor-α，TNF-α）对髓核细胞的基质金属蛋白酶-28（matrix metalloproteinase-28， MMP-28）是否具有调控作用。方法培养人体正常髓核细胞，将3个不同浓度的IL-1β（0 ng/mL、10 ng/mL、50 ng/mL）和3个不同浓度的TNF-α（0 ng/mL、50 ng/mL、100 ng/mL）分别与体外扩增的第3代髓核细胞联合培养72 h，采用实时聚合酶链反应（real time-polymerase chain reaction ， RT-PCR）法测定MMP-28 mRNA的转录水平，采用单因素方差分析法比较转录水平的差异。结果不同浓度（0 ng/mL、10 ng/mL、50 ng/mL）的IL-1β与髓核细胞共培养72 h后，各组间MMP-28mRNA转录水平（0．325±0．019、0．343±0．018、0．343±0．018）差异无统计学意义（P＞0．05）。不同浓度（0 ng/mL、50 ng/mL、100 ng/mL）TNF-α与髓核细胞共培养72 h后，各组间MMP-28 mRNA转录水平（0．325±0．019、0．515±0．02、0．610±0．012）差异有统计学意义（P＜0．01），且高浓度组MMP-28 mRNA转录水平较低浓度组明显升高（P＜0．01）。结论 IL-1β对正常人体髓核细胞MMP-28转录无调控作用，而TNF-α可以上调MMP-28mRNA转录水平，且呈浓度依赖性。
목적：탐토백세포개소-1β（ interleukin-1β，IL-1β）、종류배사인자-α（ tumor necrosis factor-α，TNF-α）대수핵세포적기질금속단백매-28（matrix metalloproteinase-28， MMP-28）시부구유조공작용。방법배양인체정상수핵세포，장3개불동농도적IL-1β（0 ng/mL、10 ng/mL、50 ng/mL）화3개불동농도적TNF-α（0 ng/mL、50 ng/mL、100 ng/mL）분별여체외확증적제3대수핵세포연합배양72 h，채용실시취합매련반응（real time-polymerase chain reaction ， RT-PCR）법측정MMP-28 mRNA적전록수평，채용단인소방차분석법비교전록수평적차이。결과불동농도（0 ng/mL、10 ng/mL、50 ng/mL）적IL-1β여수핵세포공배양72 h후，각조간MMP-28mRNA전록수평（0．325±0．019、0．343±0．018、0．343±0．018）차이무통계학의의（P＞0．05）。불동농도（0 ng/mL、50 ng/mL、100 ng/mL）TNF-α여수핵세포공배양72 h후，각조간MMP-28 mRNA전록수평（0．325±0．019、0．515±0．02、0．610±0．012）차이유통계학의의（P＜0．01），차고농도조MMP-28 mRNA전록수평교저농도조명현승고（P＜0．01）。결론 IL-1β대정상인체수핵세포MMP-28전록무조공작용，이TNF-α가이상조MMP-28mRNA전록수평，차정농도의뢰성。
Objective To investigate whether matrix metauoproteirase-28 ( MMP-28 ) can be regulated by interleubin-β( IL-1β) and tumor necrcsis factor-α( TNF-α) in human nucleus pulposus cells after coculture .Methods After the third gen-eration of human nucleus pulposus cells were stimulated with different concentration of IL -1β(0 ng/mL,10 ng/mL,50 ng/mL) and TNF-α(0 ng/mL,50 ng/mL,100 ng/mL) 72 h in vitro, MMP-28 mRNA was quantified by RT-PCR,the difference between each concentration were compared by one-way analysis of variance .Results There was no sign indicated that the various concentration of IL-1β(0 ng/mL,10 ng/mL,50 ng/mL) can affect human nucleus pulposus cells MMP-28 transcrip-tion (0.325 ±0.019 vs.0.343 ±0.018 vs.0.343 ±0.018, P>0.05).But cytokines TNF-αcould induce the expression of MMP-28, various concentration groups present significant difference (0.325 ±0.019 vs.0.515 ±0.02 vs.0.610 ±0.012, P<0.01), and with the concentration increasing , the effect was more prominent (0.610 ±0.012 vs.0.515 ±0.02, P<0.01).Conclusion IL-1βdoesn’t affect human nucleus pulposus cells MMP-28 transcription in vitro.However, TNF-αcan up regulate the MMP-28 mRNA of the nucleus pulposus cells and the effects are strengthened by enhancing concentration .