中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
8期
1532-1536
,共5页
胡曦文%章倩倩%雷岩%刘红英%周大磊%陈佳园%王丽京
鬍晞文%章倩倩%雷巖%劉紅英%週大磊%陳佳園%王麗京
호희문%장천천%뢰암%류홍영%주대뢰%진가완%왕려경
转基因小鼠%p110δ%ApcMin/+小鼠
轉基因小鼠%p110δ%ApcMin/+小鼠
전기인소서%p110δ%ApcMin/+소서
Transgenicmice%p110δ%ApcMin/+mice
目的：建立p110δ突变失活的ApcMin/＋结直肠癌癌前病变小鼠模型，为研究p110δ在小鼠结直肠癌癌前病变中的作用提供实验模型。方法：将C57BL/6J背景的ApcMin/＋结直肠癌癌前病变小鼠与p110δ突变失活小鼠（p110δD910A/D910A）进行杂交建系，通过PCR技术鉴定子代小鼠基因型，获得p110δ突变失活的ApcMin/＋小鼠。对适龄小鼠进行肠道取材，亚甲蓝染色后，观察肠道结构，对腺瘤和微腺瘤进行统计。肠道组织进行石蜡包埋、切片，做HE染色进行进一步观察。结果：获得p110δ突变失活的ApcMin/＋杂交鼠（ ApcMin/＋；p110δD910A/D910A ）并得以稳定传代。 ApcMin/＋；p110δD910A/D910A杂交小鼠的肠道组织中，腺瘤数目和体积比ApcMin/＋结直肠癌癌前病变小鼠均有减少。结论：成功建立p110δ突变失活的ApcMin/＋结直肠癌癌前病变小鼠模型，并得到小鼠肠道肿瘤的初步表型，为进一步研究p110δ在肠道肿瘤发生发展中的作用提供重要的工具动物。
目的：建立p110δ突變失活的ApcMin/＋結直腸癌癌前病變小鼠模型，為研究p110δ在小鼠結直腸癌癌前病變中的作用提供實驗模型。方法：將C57BL/6J揹景的ApcMin/＋結直腸癌癌前病變小鼠與p110δ突變失活小鼠（p110δD910A/D910A）進行雜交建繫，通過PCR技術鑒定子代小鼠基因型，穫得p110δ突變失活的ApcMin/＋小鼠。對適齡小鼠進行腸道取材，亞甲藍染色後，觀察腸道結構，對腺瘤和微腺瘤進行統計。腸道組織進行石蠟包埋、切片，做HE染色進行進一步觀察。結果：穫得p110δ突變失活的ApcMin/＋雜交鼠（ ApcMin/＋；p110δD910A/D910A ）併得以穩定傳代。 ApcMin/＋；p110δD910A/D910A雜交小鼠的腸道組織中，腺瘤數目和體積比ApcMin/＋結直腸癌癌前病變小鼠均有減少。結論：成功建立p110δ突變失活的ApcMin/＋結直腸癌癌前病變小鼠模型，併得到小鼠腸道腫瘤的初步錶型，為進一步研究p110δ在腸道腫瘤髮生髮展中的作用提供重要的工具動物。
목적：건립p110δ돌변실활적ApcMin/＋결직장암암전병변소서모형，위연구p110δ재소서결직장암암전병변중적작용제공실험모형。방법：장C57BL/6J배경적ApcMin/＋결직장암암전병변소서여p110δ돌변실활소서（p110δD910A/D910A）진행잡교건계，통과PCR기술감정자대소서기인형，획득p110δ돌변실활적ApcMin/＋소서。대괄령소서진행장도취재，아갑람염색후，관찰장도결구，대선류화미선류진행통계。장도조직진행석사포매、절편，주HE염색진행진일보관찰。결과：획득p110δ돌변실활적ApcMin/＋잡교서（ ApcMin/＋；p110δD910A/D910A ）병득이은정전대。 ApcMin/＋；p110δD910A/D910A잡교소서적장도조직중，선류수목화체적비ApcMin/＋결직장암암전병변소서균유감소。결론：성공건립p110δ돌변실활적ApcMin/＋결직장암암전병변소서모형，병득도소서장도종류적초보표형，위진일보연구p110δ재장도종류발생발전중적작용제공중요적공구동물。
[ABSTRACT]AIM:Toestablishatransgenicheterozygousmousemodelofprecancerouslesionsofcolorectal cancer with p110δmutation in the C57BL/6J background for serving the studies on colorectal cancer research mediated by p110δ.METHODS:The transgenic heterozygous mice were generated by crossing in p110δD910A/D910A mouse and ApcMin/+mouse, and the genotype was detected by PCR .Compared with ApcMin/+mice, transgenic heterozygous mice ( ApcMin/+;p110δD910A/D910A)were counted, and the number and size of intestine polyps were analyzed after methylene blue staining . The intestinal tissue structure was assessed by HE staining .RESULTS:The transgenic heterozygous mouse model of pre-cancerous lesions of colorectal cancer with p 110δmutation was established .The number and size of polyps in the transgenic heterozygous mice were declined .CONCLUSION: A transgenic heterozygous mouse model of precancerous lesions of colorectal cancer with p 110δmutation was successfully established .The initial phenotype of intestinal tumors in transgenic mice was observed .This model will greatly contribute to the related research of colorectal cancer in mice .
