CAJ | 학술논문

目的：研究冬凌草甲素对人肺癌NCI-H460细胞侵袭和迁移的影响。方法：将NCI-H460细胞分为高剂量（ HD）组、中剂量（ MD）组、低剂量（ LD）组和对照（ N）组，前3组（实验组）加入不同浓度的冬凌草甲素，N组不加冬凌草甲素，观察细胞生长情况；用MTT法测定细胞增殖；比较细胞侵袭能力和迁移能力；Western blotting检测细胞MMP-2和MMP-9表达。结果：实验组细胞数量低于对照组，细胞增殖受抑制，HD组抑制率为48．94％， MD组为36．17％，LD组为19．15％；实验组穿透滤膜细胞数明显减少，HD组为26．67±5．16，MD组为36．17±5．08，LD组为44．33±5．50；实验组的迁移速率及迁移细胞数均降低；实验组的MMP-2和MMP-9表达水平明显低于N组（P＜0．01）。结论：冬凌草甲素能抑制NCI-H460肺癌细胞的侵袭和迁移，其作用与下调MMP-2和MMP-9表达有关。
목적：연구동릉초갑소대인폐암NCI-H460세포침습화천이적영향。방법：장NCI-H460세포분위고제량（ HD）조、중제량（ MD）조、저제량（ LD）조화대조（ N）조，전3조（실험조）가입불동농도적동릉초갑소，N조불가동릉초갑소，관찰세포생장정황；용MTT법측정세포증식；비교세포침습능력화천이능력；Western blotting검측세포MMP-2화MMP-9표체。결과：실험조세포수량저우대조조，세포증식수억제，HD조억제솔위48．94％， MD조위36．17％，LD조위19．15％；실험조천투려막세포수명현감소，HD조위26．67±5．16，MD조위36．17±5．08，LD조위44．33±5．50；실험조적천이속솔급천이세포수균강저；실험조적MMP-2화MMP-9표체수평명현저우N조（P＜0．01）。결론：동릉초갑소능억제NCI-H460폐암세포적침습화천이，기작용여하조MMP-2화MMP-9표체유관。
[ABSTRACT]AIM:ToinvestigatetheeffectoforidoninontheinvasionandmigrationofhumanlungcancerNCI-H460 cells.METHODS:NCI-H460 cells were divided into high-dose (HD), middle-dose (MD) and low-dose (LD) oridonin groups (cultured with 40, 20 and 10μmol/L of oridonin, respectively, as experimental groups), and normal (N) group ( treated without oridonin as control ) .The cell growth was observed .The cell proliferation was detected by MTT as-say.Boyden chamber was used to determine the cell invasive capacity .The cell migration was also measured .The levels of MMP-2 and MMP-9 were assayed by Western blotting .RESULTS:The cell counts in the experimental groups were lower than that in N group .The cell proliferation was inhibited as the inhibitory rates were 48.94%, 36.17%and 19.15% for HD group, MD group and LD group, respectively.The numbers of the invasive cells were 26.67 ±5.16 for HD group, 36.17 ±5.08 for MD group, and 44.33 ±5.50 for LD group.The migration rates in the experimental groups were lower than that in N group .The expression of MMP-2 and MMP-9 decreased dependent on the oridonin dose as follows: HD group <MD group <LD group <N group.CONCLUSION:Oridonin inhibits the invasion and migration of NCI-H460 lung cancer cells, and reduces the expression of MMP-2 and MMP-9.