CAJ | 학술논문

目的：探讨外源性CCN1对大鼠骨髓间充质干细胞（BMSCs）生长和迁移的作用。方法：构建带有绿色荧光蛋白的大鼠CCN1慢病毒载体（Lenti-GFP-CCN1）并感染大鼠BMSCs，筛选最适感染复数（MOI）；实验分为空白组、感染Lenti-GFP组和感染Lenti-GFP-CCN1组，倒置荧光显微镜下观察BMSCs感染后荧光表达情况；MTT法检测细胞存活率，划痕愈合实验检测细胞迁移作用。结果：与空白组和阴性对照组相比，Lenti-GFP-CCN1感染大鼠BMSCs后，细胞的存活率未见明显差异；感染组细胞划痕愈合实验的愈合宽度/原宽度值与空白组及阴性对照组相比差异显著（P＜0．05）。结论：外源性CCN1对正常大鼠BMSCs存活率无影响，可促进BMSCs迁移。
목적：탐토외원성CCN1대대서골수간충질간세포（BMSCs）생장화천이적작용。방법：구건대유록색형광단백적대서CCN1만병독재체（Lenti-GFP-CCN1）병감염대서BMSCs，사선최괄감염복수（MOI）；실험분위공백조、감염Lenti-GFP조화감염Lenti-GFP-CCN1조，도치형광현미경하관찰BMSCs감염후형광표체정황；MTT법검측세포존활솔，화흔유합실험검측세포천이작용。결과：여공백조화음성대조조상비，Lenti-GFP-CCN1감염대서BMSCs후，세포적존활솔미견명현차이；감염조세포화흔유합실험적유합관도/원관도치여공백조급음성대조조상비차이현저（P＜0．05）。결론：외원성CCN1대정상대서BMSCs존활솔무영향，가촉진BMSCs천이。
[ABSTRACT]AIM:Toinvestigatetheroleofcysteine-rich61(Cyr61/CNN1)inproliferationandmigrationof bone marrow mesenchymal stem cells ( BMSCs ) .METHODS: The lentiviral vector carrying CCN 1 ( Lenti-GFP-CCN1 ) was constructed and then transfected into the rat BMSCs .The cells were divided into non-transfection group , transfection group ( transfected with Lenti-GFP-CCN1 ) and negative control group ( Lenti-GFP ) .The fluorescence intensity of the transfected BMSCs was observed under inverted fluorescence microscope .The effects of CCN1 on the proliferation and mi-gration of BMSCs were detected by MTT assay and scratch wound healing assay .RESULTS:The proliferation of BMSCs transfected with Lenti-GFP CCN1 had no significant difference compared with negative control group and control group .The width/thickness ratio of migrated BMSCs in wound healing was significantly higher in Lenti-GFP-CCN1 group than that in negative control group and control group (P<0.05).CONCLUSION:Exogenous CCN1 promotes the migration of BMSCs.