CAJ | 학술논문

目的：观察法尼酯衍生物X受体（farnesoidXreceptor，FXR）-促甲状腺素胚胎因子（thyrotropinem-bryonic factor ，TEF）通路在自身免疫性肝炎模型小鼠肝损害中的作用，探讨FXR-TEF通路改善自身免疫性肝炎的部分可能机制。方法：检测FXR在伴刀豆球蛋白A （ concanavalin A ， Con A）诱导的肝炎（ Con A-induced hepatitis ， CIH）小鼠肝脏的表达；检测FXR激活对TEF表达的影响；观察C57BL/6小鼠和鹅去氧胆酸（ chenodeoxycholic acid ， CDCA）激活FXR的CIH小鼠肝脏病理、肝脏酶学及炎症因子变化。结果：FXR在CIH小鼠中低表达；CDCA激活FXR的C57BL/6小鼠TEF表达上调；FXR被激活的CIH小鼠的肝损害较轻，FXR激活可减轻肝脏炎症因子释放。结论：CDCA激活FXR能减轻CIH引起的肝功能损害和炎症反应。 FXR激活使TEF上调。 FXR可能是自身免疫性肝炎的保护因素，其保护作用可能是通过TEF来实现的。激活FXR可能成为治疗自身免疫性肝炎的一个途径。
목적：관찰법니지연생물X수체（farnesoidXreceptor，FXR）-촉갑상선소배태인자（thyrotropinem-bryonic factor ，TEF）통로재자신면역성간염모형소서간손해중적작용，탐토FXR-TEF통로개선자신면역성간염적부분가능궤제。방법：검측FXR재반도두구단백A （ concanavalin A ， Con A）유도적간염（ Con A-induced hepatitis ， CIH）소서간장적표체；검측FXR격활대TEF표체적영향；관찰C57BL/6소서화아거양담산（ chenodeoxycholic acid ， CDCA）격활FXR적CIH소서간장병리、간장매학급염증인자변화。결과：FXR재CIH소서중저표체；CDCA격활FXR적C57BL/6소서TEF표체상조；FXR피격활적CIH소서적간손해교경，FXR격활가감경간장염증인자석방。결론：CDCA격활FXR능감경CIH인기적간공능손해화염증반응。 FXR격활사TEF상조。 FXR가능시자신면역성간염적보호인소，기보호작용가능시통과TEF래실현적。격활FXR가능성위치료자신면역성간염적일개도경。
[ABSTRACT]AIM:ToobservehowfarnesoidXreceptor(FXR)functionedinconcanavalinA(ConA)-induced hepatitis (CIH) and the regulation of FXR-thyrotropin embryonic factor (TEF) pathway.METHODS:C57BL/6 mice were injected with Con A to induce hepatitis .The expression of FXR and TEF in the liver specimens was determined by qRT-PCR and Western blotting .The concentrations of serum ALT/AST and inflammatory cytokines IFN-γ, TNF-α, IL-4 and IL-2 in the blood samples were tested after Con A injection .RESULTS:FXR was down-regulated in CIH mice .TEF was up-regula-ted when FXR was activated by chenodeoxycholic acid (CDCA).Activation of FXR reduced the levels of aminotransferases and inflammatory cytokines IFN-γ, TNF-α, IL-4 and IL-2 in the CIH mice induced by Con A injection .CONCLUSION:FXR activation attenuates CIH mouse liver injury and reduces inflammatory cytokines .FXR activation results in TEF up-regu-lation.The FXR-TEF pathway may play a protective role in autoimmune hepatitis .