中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
8期
1421-1426
,共6页
于杨%冯玉梅%郭守东%崔英杰%宋国华%冯蕾%罗甜%陈超%王义维%蒋宪成%秦树存
于楊%馮玉梅%郭守東%崔英傑%宋國華%馮蕾%囉甜%陳超%王義維%蔣憲成%秦樹存
우양%풍옥매%곽수동%최영걸%송국화%풍뢰%라첨%진초%왕의유%장헌성%진수존
鞘氨醇-1-磷酸%磷脂转运蛋白%脂蛋白类, HDL%载脂蛋白M%红细胞
鞘氨醇-1-燐痠%燐脂轉運蛋白%脂蛋白類, HDL%載脂蛋白M%紅細胞
초안순-1-린산%린지전운단백%지단백류, HDL%재지단백M%홍세포
Sphingosine-1-phosphate%Phospholipid transfer proteins%Lipoproteins,HDL%Apolipoprotein M%Erythrocytes
目的：探讨血浆脂蛋白上鞘氨醇-1-磷酸（S1P）和磷脂转运蛋白（PLTP）的相互作用及机制。方法：检测雄性10周龄PLTP转基因（PLTP-Tg）及野生型（WT）小鼠血浆S1P和脂蛋白上S1P含量，转运实验检测PLTP对S1P的转运作用，免疫印迹检测S1P载体载脂蛋白M（ apoM）含量。结果：PLTP-Tg小鼠血浆S1P含量较WT小鼠下降21．1％（P＜0．01），高密度脂蛋白（HDL）组分上S1P含量下降约35．1％，而低密度脂蛋白（LDL）组分上S1P比例则增加了127．4％。转运实验表明，在37℃时D-Hanks 缓冲液中PLTP能够促进S1P从红细胞转运至重组脂质体。 PLTP-Tg小鼠血浆中apoM含量与WT小鼠比较无变化。结论：生理水平PLTP对保持HDL上S1P含量有重要意义，过表达PLTP可显著降低HDL上S1P，同时升高LDL上S1P，其机制可能与PLTP介导S1P转运有关。
目的：探討血漿脂蛋白上鞘氨醇-1-燐痠（S1P）和燐脂轉運蛋白（PLTP）的相互作用及機製。方法：檢測雄性10週齡PLTP轉基因（PLTP-Tg）及野生型（WT）小鼠血漿S1P和脂蛋白上S1P含量，轉運實驗檢測PLTP對S1P的轉運作用，免疫印跡檢測S1P載體載脂蛋白M（ apoM）含量。結果：PLTP-Tg小鼠血漿S1P含量較WT小鼠下降21．1％（P＜0．01），高密度脂蛋白（HDL）組分上S1P含量下降約35．1％，而低密度脂蛋白（LDL）組分上S1P比例則增加瞭127．4％。轉運實驗錶明，在37℃時D-Hanks 緩遲液中PLTP能夠促進S1P從紅細胞轉運至重組脂質體。 PLTP-Tg小鼠血漿中apoM含量與WT小鼠比較無變化。結論：生理水平PLTP對保持HDL上S1P含量有重要意義，過錶達PLTP可顯著降低HDL上S1P，同時升高LDL上S1P，其機製可能與PLTP介導S1P轉運有關。
목적：탐토혈장지단백상초안순-1-린산（S1P）화린지전운단백（PLTP）적상호작용급궤제。방법：검측웅성10주령PLTP전기인（PLTP-Tg）급야생형（WT）소서혈장S1P화지단백상S1P함량，전운실험검측PLTP대S1P적전운작용，면역인적검측S1P재체재지단백M（ apoM）함량。결과：PLTP-Tg소서혈장S1P함량교WT소서하강21．1％（P＜0．01），고밀도지단백（HDL）조분상S1P함량하강약35．1％，이저밀도지단백（LDL）조분상S1P비례칙증가료127．4％。전운실험표명，재37℃시D-Hanks 완충액중PLTP능구촉진S1P종홍세포전운지중조지질체。 PLTP-Tg소서혈장중apoM함량여WT소서비교무변화。결론：생리수평PLTP대보지HDL상S1P함량유중요의의，과표체PLTP가현저강저HDL상S1P，동시승고LDL상S1P，기궤제가능여PLTP개도S1P전운유관。
[ABSTRACT]AIM:Toinvestigatetheinteractionandthemechanismofsphingosine-1-phosphate(S1P)and phospholipid transfer protein (PLTP) in lipoprotein.METHODS:The S1P content in the plasma and lipoprotein from 10-week-old PLTP transgenic (PLTP-Tg) mice and wild-type (WT) mice (n=8 each) was assayed.The transport of S1P by PLTP was determined by S1P transfer assay.The content of specific S1P carrier, apolipoprotein M, was detected by West-ern blotting.RESULTS:Plasma S1P contents were decreased by 21.1%in PLTP-Tg mice compared with WT mice.S1P content in high-density lipoprotein ( HDL) fraction ( HDL-S1P) from PLTP-Tg mice was decreased by 35.1% compared with WT mice, whereas the S1P in low-density lipoprotein (LDL) fraction (LDL-S1P) was increased by 127.4%.The re-sults of S1P transfer assay indicated that PLTP facilitated S 1P transport from erythrocyte to recombinant liposome at 37℃in D-Hanks buffer solution .The plasma content of apolipoprotein M was not changed in PLTP-Tg mice compared with WT mice.CONCLUSION:PLTP is a key factor to maintain plasma HDL-S1P under physical condition .Overexpression of PLTP decreases the HDL-S1P but increases LDL-S1P.The mechanism might be related to the capability of PLTP on trans-ferring S1P from erythrocyte to lipoprotein.
