CAJ | 학술논문

目的：探讨白藜芦醇抑制软骨肉瘤的机制及对线粒体途径和PI3K/Akt通路的影响。方法：SW1353软骨肉瘤细胞培养至对数生长期后设对照组和白藜芦醇处理组，药物处理组用25、50和100μmol/L白藜芦醇处理24 h、48 h或72 h。采用CCK8法检测SW1353软骨肉瘤细胞的活力，Hoechst 33258荧光染色观察细胞凋亡，Western blotting检测activated caspase-3、Bcl-2、Bax、Akt和p-Akt蛋白在细胞中表达情况，细胞划痕实验观察细胞迁移情况。结果：白藜芦醇处理后细胞活力下降，呈时间-剂量依赖性（P＜0．01）。 Hoechst 33258染色检测可见药物处理组有明显的细胞凋亡核象。 Western blotting 检测显示药物处理组activated caspase-3和Bax蛋白表达上调，Bcl-2蛋白和p-Akt蛋白表达下调，总Akt改变不显著。细胞划痕试验显示，白藜芦醇能显著抑制SW1353细胞的迁移。结论：白藜芦醇能够诱导软骨肉瘤凋亡，部分是通过线粒体途径及PI3K/Akt信号通路发挥作用。
목적：탐토백려호순억제연골육류적궤제급대선립체도경화PI3K/Akt통로적영향。방법：SW1353연골육류세포배양지대수생장기후설대조조화백려호순처리조，약물처리조용25、50화100μmol/L백려호순처리24 h、48 h혹72 h。채용CCK8법검측SW1353연골육류세포적활력，Hoechst 33258형광염색관찰세포조망，Western blotting검측activated caspase-3、Bcl-2、Bax、Akt화p-Akt단백재세포중표체정황，세포화흔실험관찰세포천이정황。결과：백려호순처리후세포활력하강，정시간-제량의뢰성（P＜0．01）。 Hoechst 33258염색검측가견약물처리조유명현적세포조망핵상。 Western blotting 검측현시약물처리조activated caspase-3화Bax단백표체상조，Bcl-2단백화p-Akt단백표체하조，총Akt개변불현저。세포화흔시험현시，백려호순능현저억제SW1353세포적천이。결론：백려호순능구유도연골육류조망，부분시통과선립체도경급PI3K/Akt신호통로발휘작용。
[ABSTRACT]AIM:Toinvestigatetheinhibitoryeffectsofresveratrolonchondrosarcomaandtherelationwith mitochondrial and PI3K/Akt pathways.METHODS:Chondrosarcoma SW1353 cells were treated with resveratrol at con-centrations of 25, 50 and 100 μmol/L for the time intervals of 24 h, 48 h and 72 h.The viability and apoptosis of the SW1353 cells in the presence or absence of resveratrol were analyzed by CCK 8 assay and Hoechst 33258 staining , respec-tively.The protein levels of Bcl-2, Bax, activated caspase-3, Akt and p-Akt were detected by Western blotting .The cell migration ability was determined by wound scratch assay .RESULTS:Exposure of the cells to resveratrol resulted in a de-crease in the cell viability in a dose-and time-dependent manner (P<0.05).visible nuclei with apoptotic characteristics in resveratrol group were observed .The protein levels of activated caspase-3 and Bax were increased , and Bcl-2 and p-Akt were decreased compared with control group .The total Akt were not significantly changed .Resveratrol also significantly re-duced the migration of tumor cells .CONCLUSION:Resveratrol induces apoptosis of chondrosarcoma , which plays a role of part through mitochondrial and PI 3K/Akt signaling pathways .