CAJ | 학술논문

目的：研究miR-155特异性siRNA增强阿糖胞苷（Ara-C）诱导的Burkitt淋巴瘤Raji细胞凋亡及其机制。方法：Ara-C与miR-155 siRNA单独或者联合干预Raji细胞增殖。 qRT-PCR检测脂质体转染siRNA后miR-155的表达；CCK-8法检测细胞增殖；流式细胞术检测细胞凋亡；Western blotting 法检测caspase-3的表达。结果：qRT-PCR显示，转染miR-155 siRNA后，Raji细胞的miR-155表达明显下降（P＜0．05），Ara-C或miR-155 siRNA单独处理细胞后均显示出增殖抑制作用，而两者联合使用的增殖抑制作用更显著（ P＜0．05）。各组细胞进行药物干预48 h后行流式细胞术，结果显示Ara-C或miR-155 siRNA单独处理细胞凋亡率分别为（16．5±0．3）％和（14．6±0．3）％，与对照组[（3．6±0．4）％]比较差别有统计学意义（P＜0．05），Ara-C＋miR-155 siRNA组的凋亡率[（38．4±1．4）％]分别与Ara-C和miR-155 siRNA单独处理组比较，差别有统计学意义（ P＜0．05）。 Ara-C ＋miR-155 siRNA组与Ara-C组及miR-155 siRNA组相比，caspase-3的水平明显增高。结论： miR-155特异性siRNA具有增强阿糖胞苷对Raji细胞增殖抑制及诱导Raji细胞凋亡的作用，其机制与caspase-3凋亡途径有关。
목적：연구miR-155특이성siRNA증강아당포감（Ara-C）유도적Burkitt림파류Raji세포조망급기궤제。방법：Ara-C여miR-155 siRNA단독혹자연합간예Raji세포증식。 qRT-PCR검측지질체전염siRNA후miR-155적표체；CCK-8법검측세포증식；류식세포술검측세포조망；Western blotting 법검측caspase-3적표체。결과：qRT-PCR현시，전염miR-155 siRNA후，Raji세포적miR-155표체명현하강（P＜0．05），Ara-C혹miR-155 siRNA단독처리세포후균현시출증식억제작용，이량자연합사용적증식억제작용경현저（ P＜0．05）。각조세포진행약물간예48 h후행류식세포술，결과현시Ara-C혹miR-155 siRNA단독처리세포조망솔분별위（16．5±0．3）％화（14．6±0．3）％，여대조조[（3．6±0．4）％]비교차별유통계학의의（P＜0．05），Ara-C＋miR-155 siRNA조적조망솔[（38．4±1．4）％]분별여Ara-C화miR-155 siRNA단독처리조비교，차별유통계학의의（ P＜0．05）。 Ara-C ＋miR-155 siRNA조여Ara-C조급miR-155 siRNA조상비，caspase-3적수평명현증고。결론： miR-155특이성siRNA구유증강아당포감대Raji세포증식억제급유도Raji세포조망적작용，기궤제여caspase-3조망도경유관。
[ABSTRACT]AIM:ToinvestigatetheeffectofmiR-155-specificsiRNAaloneorincombinationwithcytosinear-abinoside (Ara-C) on the growth and apoptosis of Burkitt lymphoma Raji cells .METHODS: miR-155-specific siRNA and/or Ara-C were used to treat the cells .Quantitative real-time polymerase chain reaction was used to detect the expres-sion of miR-155.The growth of the cells was analyzed by CKK-8 assay.The cell apoptosis was determined by flow cytome-try.RESULTS:The miR-155 expression level of the cells transfected with miR-155 siRNA was significantly lower than that in the 2 control groups .Ara-C or miR-155 siRNA alone inhibited the growth of Raji cells in a dose-depend manner . miR-155 siRNA combined with Ara-C produced more inhibition of cell proliferation (P<0.05).After treatment for 48 h, the apoptotic rate of Raji cells in miR-155 siRNA+Ara-C group [(38.4 ±1.4)%] was higher than that in Ara-C group [(16.5 ±0.3)%] and miR-155 siRNA group [(14.6 ±0.3)%], with statistically significant difference (P<0.05). The expression of caspase-3 in Ara-C+miR-155 siRNA group was increased significantly as compared with Ara-C group and miR-155 siRNA group.CONCLUSION:miR-155-specific siRNA enhances the chemosensitivity of Raji cells to Ara-C by inducing apoptosis through the caspase-3 pathway .