CAJ | 학술논문

目的：探讨超声引导下经皮瘤内注射无水乙醇（percutaneous alcohol injection therapy ，PEIT ）联合榄香烯注射液静脉给药治疗兔移植性VX2肝肿瘤的疗效及作用机理。方法建立兔移植性VX2肝肿瘤模型30只，随机分为2组：经皮瘤内注射无水乙醇联合榄香烯注射液静脉滴注治疗组（15只）；单纯经皮无水乙醇注射治疗组（15只）。实验动物在接种15天后每隔3天治疗一次，5次后处死。手术前、后抽兔耳缘静脉血行血生化检查，M SC T 扫描测量肿瘤大小变化，同时进行组织细胞学与细胞超微结构的观察。结果 PEIT＋榄香烯注射液治疗组肿瘤增长率与坏死率与单纯PEIT治疗组比较差异均有统计学意义（ P ＜0．05）；TUNEL染色法检测显示PEIT＋榄香烯注射液治疗组肿瘤细胞凋亡指数明显高于单纯PEIT治疗组（ P ＜0．05）；PEIT＋榄香烯注射液治疗组血管内皮生长因子（VEGF）表达水平明显低于单纯PEIT 治疗组（ P ＜0．05）；前者2例出现肝内转移灶，而后者5例出现转移灶，差异性有显著意义（ P ＜0．05）；治疗前后两治疗组血生化及肝肾功检查均差异无显著性意义（ P ＞0．05）。结论 PEIT联合榄香烯注射液治疗兔移植性VX2肝肿瘤可明显诱导肿瘤细胞凋亡、抑制VEGF的表达以及肝内转移。
목적：탐토초성인도하경피류내주사무수을순（percutaneous alcohol injection therapy ，PEIT ）연합람향희주사액정맥급약치료토이식성VX2간종류적료효급작용궤리。방법건립토이식성VX2간종류모형30지，수궤분위2조：경피류내주사무수을순연합람향희주사액정맥적주치료조（15지）；단순경피무수을순주사치료조（15지）。실험동물재접충15천후매격3천치료일차，5차후처사。수술전、후추토이연정맥혈행혈생화검사，M SC T 소묘측량종류대소변화，동시진행조직세포학여세포초미결구적관찰。결과 PEIT＋람향희주사액치료조종류증장솔여배사솔여단순PEIT치료조비교차이균유통계학의의（ P ＜0．05）；TUNEL염색법검측현시PEIT＋람향희주사액치료조종류세포조망지수명현고우단순PEIT치료조（ P ＜0．05）；PEIT＋람향희주사액치료조혈관내피생장인자（VEGF）표체수평명현저우단순PEIT 치료조（ P ＜0．05）；전자2례출현간내전이조，이후자5례출현전이조，차이성유현저의의（ P ＜0．05）；치료전후량치료조혈생화급간신공검사균차이무현저성의의（ P ＞0．05）。결론 PEIT연합람향희주사액치료토이식성VX2간종류가명현유도종류세포조망、억제VEGF적표체이급간내전이。
Objective To explore the principle ,effect and method of PEIT combined with elemene injection for tumor ablation .Methods Thirty new zealand white rabbits with implanted liver VX2 tumors were randomly divided into two groups:PEIT + elemene injection group (n=15) ,percutaneous ethanol injection (PEI) group (n=15) .Fifty days after transplantation ,the drugs were injected into tumors of all the rabbits once every 3 days .After 5 times of injection ,the rabbits were killed .The tumors were measured 3 dimensionally with CT ,while the rates of tumor necrosis ,the volume of tumor and the growth rate of the tumor were calculated .Liver and kidney function tests (AST ,ALT ,ALP and BUN) as well as white blood cell counts were assessed before and after treatment ,and CT examination was performed at the same time .The morphological and the ultramicrostructural changes were also investigated under light microscope or electron mi-croscope .Apoptotic cells were evaluated with TUNEL essay ,in the same time , vascular endothelial growth factor (VEGF) and expression were examined by immunohistochemistry .Results Among all 2 groups ,tumor growing rate for PEIT + elemene injection group was the lowest and the necrotic rate was the highest ( P<0 .05) .Apoptotic index (AI) in PEIT + elemene injection group was significantly higher than that in PEIT group a ( P <0 .05) .The expression of VEGF protein was statistically lowest of the AiDi injection group ( P <0 .05) .There was no evident change of liver and kidney function before and after treatment in the 2 groups ( P>0 .05) .Conclusion PEIT + elemene injection can induce apoptosis of liver VX2 tumor cells obviously ,and inhibit the expression of VEGF of in the liver VX2 tumors tissue .