CAJ | 학술논문

目的：采用高效液相色谱法测定8种茶叶中咖啡因的含量。方法采用 Ultimate XB-C18（4.6×250mm，5μm）色谱柱，流动相：乙腈-0.2％磷酸水溶液，梯度洗脱，流速1.0mL· min -1，检测波长为280nm，进样量20μL。结果在所选条件下，样品中待测组分与其它组分分离良好（R＞1.5），咖啡因对照品在10～100μg· mL -1质量浓度范围内线性关系良好，r＝0.9998；回收率为98.8％～102.5％，RSD＝1.6％（n＝6）。结论该方法操作简便，结果可靠，适合用于茶叶中咖啡因定量分析。
목적：채용고효액상색보법측정8충다협중가배인적함량。방법채용 Ultimate XB-C18（4.6×250mm，5μm）색보주，류동상：을정-0.2％린산수용액，제도세탈，류속1.0mL· min -1，검측파장위280nm，진양량20μL。결과재소선조건하，양품중대측조분여기타조분분리량호（R＞1.5），가배인대조품재10～100μg· mL -1질량농도범위내선성관계량호，r＝0.9998；회수솔위98.8％～102.5％，RSD＝1.6％（n＝6）。결론해방법조작간편，결과가고，괄합용우다협중가배인정량분석。
OBJECTIVE To analyze the content of CAF in the eight kinds of tea by High-performance Liquid Chromatography.METHODS Using a Ultimate XB-C18 (4.6 ×250mm,5μm) as the chromatographic column, with the moving phase of acetonitrile:0.2%phosphoric acid solution gradient elution ,the velocity of flow was 1.0 mL · min -1 ,the UV detection wavelength was 280 nm,the sample dose was 20μL.RESULTS Under the selected con-dition,the aim ingredient in the samples got an ideal resolution with others (the resolution was above 1.5).Caffeineshowed good linear relationship in the concentration range of 10 ~100μg· mL-1 ,r =0.9998.The recovery rate was98.8% to 102.5%,RSD was 1.6%(n =6).CONCLUSION The method is simple and result is reliable,whichcan be used for quantitative analysis of caffeine in tea.