海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
8期
48-50
,共3页
天然牛黄%猪去氧胆酸%薄层色谱%高效液相色谱法
天然牛黃%豬去氧膽痠%薄層色譜%高效液相色譜法
천연우황%저거양담산%박층색보%고효액상색보법
Calculus bovis%Hyodeoxycholic acid%TLC%HPLC
目的：建立薄层色谱法和高效液相法监测天然牛黄中是否含有猪去氧胆酸。方法 TLC 法采用硅胶G 薄层板，以正己烷-乙酸乙酯-醋酸-甲醇（20∶25∶2∶3）的上层溶液为展开剂展开。 HPLC法采用Lichrospher C18（4.6mm ×250mm，5μm）色谱柱，以乙腈-水-磷酸（35∶65∶0.1）为流动相，检测波长192nm，流速1.0mL· min -1。结果猪去氧胆酸在0.102～5.088mg· mL -1范围内呈良好的线性关系（r＝0.9999）；加样回收率为97.1％，RSD％为1.2％。结论该法简便准确，灵敏度高，通用性好，适用于牛黄中猪去氧胆酸的监控。
目的：建立薄層色譜法和高效液相法鑑測天然牛黃中是否含有豬去氧膽痠。方法 TLC 法採用硅膠G 薄層闆，以正己烷-乙痠乙酯-醋痠-甲醇（20∶25∶2∶3）的上層溶液為展開劑展開。 HPLC法採用Lichrospher C18（4.6mm ×250mm，5μm）色譜柱，以乙腈-水-燐痠（35∶65∶0.1）為流動相，檢測波長192nm，流速1.0mL· min -1。結果豬去氧膽痠在0.102～5.088mg· mL -1範圍內呈良好的線性關繫（r＝0.9999）；加樣迴收率為97.1％，RSD％為1.2％。結論該法簡便準確，靈敏度高，通用性好，適用于牛黃中豬去氧膽痠的鑑控。
목적：건립박층색보법화고효액상법감측천연우황중시부함유저거양담산。방법 TLC 법채용규효G 박층판，이정기완-을산을지-작산-갑순（20∶25∶2∶3）적상층용액위전개제전개。 HPLC법채용Lichrospher C18（4.6mm ×250mm，5μm）색보주，이을정-수-린산（35∶65∶0.1）위류동상，검측파장192nm，류속1.0mL· min -1。결과저거양담산재0.102～5.088mg· mL -1범위내정량호적선성관계（r＝0.9999）；가양회수솔위97.1％，RSD％위1.2％。결론해법간편준학，령민도고，통용성호，괄용우우황중저거양담산적감공。
OBJECTIVE To establish a TLC and HPLC method for monitoring the hyodeoxycholic acid in cal-culus bovis.METHODS Silica G was used and developed with n-hexane-ethyl acetate-36 acetic acid-methanol(20∶25∶2∶3) in the upper solution.A Lichrospher C18 (4.6mm ×250mm,5μm) was used as the analytical column and mobile phase was acetonitrile-water-H3PO4(35∶65∶0.1) at a flow rate of 1.0mL· min -1.The UV detection wave-length was set at 192 nm.RESULTS The hyodeoxycholic acid showed a good linear relationship at a range of 0.102~5.088 mg· mL-1 ( r=0.9999 );the average recovery was 97.1%,RSD was 1.2%.CONCULSION The method is simple ,rapid and reliable for the identification of hyodeoxycholic acid in natural calculusbovis.
