海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
8期
42-44
,共3页
何丽君%陈豪%郑婉玉%翁淑琴%程心玲
何麗君%陳豪%鄭婉玉%翁淑琴%程心玲
하려군%진호%정완옥%옹숙금%정심령
高效液相色谱法%三草胶囊%熊果酸
高效液相色譜法%三草膠囊%熊果痠
고효액상색보법%삼초효낭%웅과산
HPLC%Sanchao Capsules%Ursolic acid
目的：建立HPLC法测定三草胶囊中熊果酸的含量。方法采用Acclaim120 C18（5μm，4.6×250mm）色谱柱；流动相为甲醇-0.1％甲酸（88∶12）；流速为1mL· min -1；检测波长为210nm。结果熊果酸在2.98～119.36μg· mL -1范围与峰面积呈良好的线性关系，平均加样回收率为99.2％，RSD％为1.3％。结论该法简便，快速，结果可靠，可用于三草胶囊的质量控制。
目的：建立HPLC法測定三草膠囊中熊果痠的含量。方法採用Acclaim120 C18（5μm，4.6×250mm）色譜柱；流動相為甲醇-0.1％甲痠（88∶12）；流速為1mL· min -1；檢測波長為210nm。結果熊果痠在2.98～119.36μg· mL -1範圍與峰麵積呈良好的線性關繫，平均加樣迴收率為99.2％，RSD％為1.3％。結論該法簡便，快速，結果可靠，可用于三草膠囊的質量控製。
목적：건립HPLC법측정삼초효낭중웅과산적함량。방법채용Acclaim120 C18（5μm，4.6×250mm）색보주；류동상위갑순-0.1％갑산（88∶12）；류속위1mL· min -1；검측파장위210nm。결과웅과산재2.98～119.36μg· mL -1범위여봉면적정량호적선성관계，평균가양회수솔위99.2％，RSD％위1.3％。결론해법간편，쾌속，결과가고，가용우삼초효낭적질량공제。
OBJECTIVE To establish a HPLC method for the determination of Ursolic acid in Sanchao Cap-sules.METHODS The chromatographic column was Acclaim120 C18(5μm,4.6 ×250mm).The mobile phase was Methyl alcohol-formic acid ( 88∶12 ) .The flow rate was 1 mL · min-1 and the detection wavelength was 210 nm.RESULTS The standard curves for Ursolic acid was liner in the rang of 2.98~119.36μg· mL-1 ,The av-erage recovey was ranged from 99.2%,RSD%was 1.3%.CONCLUSION This method is simple ,quick and be-lievable.It can be effectively used for the quality control of Sanchao Capsules.
