CAJ | 학술논문

目的：探讨分子水平丁苯酞对慢性低灌注大鼠基质金属蛋白酶（MMP）2和 MMP-9及淀粉样前体蛋白（β-APP）表达的影响。方法雄性Wistar大鼠72只随机分为假手术组、缺血组、丁苯酞组，每组24只。缺血组和丁苯酞组按不同时间分为7、14、30和60 d时间点，每时间点6只。用Western blot法分析各组β-APP、MMP-2和M M P-9表达的变化。结果与7 d组比较，14、30、60 dβ-A PP表达明显升高（ P＜0．05，P＜0．01）；与14 d比较，30、60 dβ-APP表达明显升高（P＜0.01）；与30 d比较，60 d MMP-2表达明显升高（P＜0．01），丁苯酞组仅30 d与60 d比较，差异有统计学意义（P＜0．01）。与假手术组比较，缺血组和丁苯酞组β-APP和MMP-2表达明显升高（P＜0．05，P＜0．01）；与缺血组比较，丁苯酞组β-APP和 MMP-2表达明显降低（P＜0．01）。结论 MMP-2与β-APP变化趋势一致，可能参与了血脑屏障的破坏以及淀粉样物质的沉积。丁苯酞可能通过减少MMP-2及β-APP表达干预淀粉样物质的沉积。
목적：탐토분자수평정분태대만성저관주대서기질금속단백매（MMP）2화 MMP-9급정분양전체단백（β-APP）표체적영향。방법웅성Wistar대서72지수궤분위가수술조、결혈조、정분태조，매조24지。결혈조화정분태조안불동시간분위7、14、30화60 d시간점，매시간점6지。용Western blot법분석각조β-APP、MMP-2화M M P-9표체적변화。결과여7 d조비교，14、30、60 dβ-A PP표체명현승고（ P＜0．05，P＜0．01）；여14 d비교，30、60 dβ-APP표체명현승고（P＜0.01）；여30 d비교，60 d MMP-2표체명현승고（P＜0．01），정분태조부30 d여60 d비교，차이유통계학의의（P＜0．01）。여가수술조비교，결혈조화정분태조β-APP화MMP-2표체명현승고（P＜0．05，P＜0．01）；여결혈조비교，정분태조β-APP화 MMP-2표체명현강저（P＜0．01）。결론 MMP-2여β-APP변화추세일치，가능삼여료혈뇌병장적파배이급정분양물질적침적。정분태가능통과감소MMP-2급β-APP표체간예정분양물질적침적。
Objective To study the effect of 3-n-butylphthalide on expression of MMP-2 ,MMP-9 andβ-APP in rats following chronic cerebral hypoperfusion at molecular level .Methods Seventy-two male Wistar rats were randomly divided into sham operation group ,ischemic group ,and bu-tylphthalide group (24 in each group) .Rats in ischemic group and butylphthalide group were di-vided into postischemic 7 ,14 ,30 ,60 d subgroups (6 in each group) .Expressions of β-APP ,MMP-2 , MMP-9 in each group were detected by Western blot .Results The β-APP expression level was significantly higher in postischemic 14 d ,30 d ,60 d subgroups than in postischemic 7 d subgroup (P<0 .05 ,P<0 .01) ,and in postischemic 30 d ,60 d subgroups than in postischemic 14 d sub-group (P< 0 .01) .The β-APP expression level differed greatly in 30 d and 60 d butylphthalide subgroups (P<0 .01) .The MMP-2 expression level was significantly higher in postischemic 60 d subgroup than in postischemic 30 d subgroup (P<0 .05 ,P<0 .01) .Theβ-APP and MMP-2 ex-pression levels were significantly higher in ischemic group and butylphthalide group than in sham operation group (P<0 .05 ,P<0 .01) ,and significantly lower in butylphthalide group than in is-chemic group (P< 0 .01) .Conclusion MMP-2 and β-APP tend to change similarly and are in-volved in blood brain barrier destruction and amyloid deposition .Butylphthalide intervenes in am-yloid deposition by downregulating the expression of MMP-2 andβ-APP .