中华老年心脑血管病杂志
中華老年心腦血管病雜誌
중화노년심뇌혈관병잡지
CHINESE JOURNAL OF GERIATRIC CARDIOVASCULAR AND CEREBROVASCULAR DISEASES
2014年
9期
971-974
,共4页
武旭东%李卫红%陶天琪%刘秀华%唐朝枢
武旭東%李衛紅%陶天琪%劉秀華%唐朝樞
무욱동%리위홍%도천기%류수화%당조추
钙网蛋白%缺氧%p38丝裂原活化蛋白激酶类%心肌梗死
鈣網蛋白%缺氧%p38絲裂原活化蛋白激酶類%心肌梗死
개망단백%결양%p38사렬원활화단백격매류%심기경사
calreticulin%anoxia%p38 mitogen-activated protein kinases%myocardial infarction
目的:探讨缺氧预处理(H PC )对于心肌钙网蛋白表达与肌浆网钙稳态的影响及其信号转导机制。方法选择SD大鼠22只,随机分为假手术组6只,模型组8只,H PC组8只。复制SD大鼠H PC和心肌梗死模型,检测左心室压力最大上升速率和最大下降速率(± dp/dtmax )、T TC法测定心肌梗死面积,差速离心法制备心肌肌浆网并鉴定其纯度,以Millipore滤过法测定肌浆网Ca2+摄取活性和肌浆网Ca2+释放速率,Western blot检测钙网蛋白、p38丝裂原活化蛋白激酶和磷酸化 p38丝裂原活化蛋白激酶水平。结果与假手术组比较,模型组+ dp/dtmax和-dp/dtmax分别下降39%和46%(P<0.05);与模型组比较,HPC组分别升高43%和59%(P<0.05),肌浆网Ca2+摄取升高[(60.38±5.76)nmol Ca2+/(mg?min) vs (31.10±3.13)nmol Ca2+/(mg?min)],肌浆网Ca2+释放降低[(32.12±1.18)nmol Ca2+/(mg?15 s) vs (39.61±1.16)nmol Ca2+/(mg?15 s),P<0.05],钙网蛋白表达和p38丝裂原活化蛋白激酶水平明显升高( P<0.05)。结论 H PC通过p38丝裂原活化蛋白激酶途径上调钙网蛋白表达,改善心肌肌浆网C a2+摄取和肌浆网C a2+释放功能、减轻细胞内钙超载而保护缺血心肌。
目的:探討缺氧預處理(H PC )對于心肌鈣網蛋白錶達與肌漿網鈣穩態的影響及其信號轉導機製。方法選擇SD大鼠22隻,隨機分為假手術組6隻,模型組8隻,H PC組8隻。複製SD大鼠H PC和心肌梗死模型,檢測左心室壓力最大上升速率和最大下降速率(± dp/dtmax )、T TC法測定心肌梗死麵積,差速離心法製備心肌肌漿網併鑒定其純度,以Millipore濾過法測定肌漿網Ca2+攝取活性和肌漿網Ca2+釋放速率,Western blot檢測鈣網蛋白、p38絲裂原活化蛋白激酶和燐痠化 p38絲裂原活化蛋白激酶水平。結果與假手術組比較,模型組+ dp/dtmax和-dp/dtmax分彆下降39%和46%(P<0.05);與模型組比較,HPC組分彆升高43%和59%(P<0.05),肌漿網Ca2+攝取升高[(60.38±5.76)nmol Ca2+/(mg?min) vs (31.10±3.13)nmol Ca2+/(mg?min)],肌漿網Ca2+釋放降低[(32.12±1.18)nmol Ca2+/(mg?15 s) vs (39.61±1.16)nmol Ca2+/(mg?15 s),P<0.05],鈣網蛋白錶達和p38絲裂原活化蛋白激酶水平明顯升高( P<0.05)。結論 H PC通過p38絲裂原活化蛋白激酶途徑上調鈣網蛋白錶達,改善心肌肌漿網C a2+攝取和肌漿網C a2+釋放功能、減輕細胞內鈣超載而保護缺血心肌。
목적:탐토결양예처리(H PC )대우심기개망단백표체여기장망개은태적영향급기신호전도궤제。방법선택SD대서22지,수궤분위가수술조6지,모형조8지,H PC조8지。복제SD대서H PC화심기경사모형,검측좌심실압력최대상승속솔화최대하강속솔(± dp/dtmax )、T TC법측정심기경사면적,차속리심법제비심기기장망병감정기순도,이Millipore려과법측정기장망Ca2+섭취활성화기장망Ca2+석방속솔,Western blot검측개망단백、p38사렬원활화단백격매화린산화 p38사렬원활화단백격매수평。결과여가수술조비교,모형조+ dp/dtmax화-dp/dtmax분별하강39%화46%(P<0.05);여모형조비교,HPC조분별승고43%화59%(P<0.05),기장망Ca2+섭취승고[(60.38±5.76)nmol Ca2+/(mg?min) vs (31.10±3.13)nmol Ca2+/(mg?min)],기장망Ca2+석방강저[(32.12±1.18)nmol Ca2+/(mg?15 s) vs (39.61±1.16)nmol Ca2+/(mg?15 s),P<0.05],개망단백표체화p38사렬원활화단백격매수평명현승고( P<0.05)。결론 H PC통과p38사렬원활화단백격매도경상조개망단백표체,개선심기기장망C a2+섭취화기장망C a2+석방공능、감경세포내개초재이보호결혈심기。
Objective To study the effect of hypoxic preconditioning (HPC) on myocardial calreti-culin (CRT ) expression and calcium homeostasis in sarcoplasmic reticulum (SR) and its signal transduction mechanism .Methods Twenty-two SD rats were randomly divided into sham opera-tion group(n=6) ,model group (n=8) ,and HPC group (n=8) .SD rat HPC model and myocardial infarction (MI) model were established .The LV ± dp/dtmax was assayed .The infarction size was measured by TTC .The SR vesicle was prepared and its purity was identified by differential cen-trifugation .The SR Ca2+ uptake and release were detected by Millipore filtration .CRT expression and p38MAPK phosphorylation were detected by Western blot .Results The LV + dp/dt max and -dp/dtmax were 39% and 46% lower in HPC group than in sham operation group (P<0 .05) , and 43% and 59% higher in HPC group than in model group (P<0 .05) .The SR Ca2+ uptake was higher and the SR Ca2+ release was lower in HPC group than in model group (P<0 .05) .The CRT expression and p38MAPK phosphorylation levels were higher in HPC group than in model group (P<0 .05) .Conclusion HPC can upregulate the Ca2+ expression ,improve the SR Ca2+ up-take and release ,and reduce the calcium overload in myocardiocytes through the p38MAPK path-way ,thus protecting the heart against ischemia injury .