CAJ | 학술논문

目的皮下注射异丙肾上腺素制备小鼠心肌纤维化模型并对其进行评价。方法将昆明种小鼠随机分为心肌纤维化模型组和溶媒对照组,每组10只。模型组小鼠皮下注射5 mg·kg-1异丙肾上腺素1 d后,以2.5 mg·kg-1维持至30 d。溶媒对照组同法皮下注射等量的生理盐水。30 d后颈椎脱臼处死小鼠,取心脏称重,计算心重指数( cardiac weight index, CWI)；消化法测定心肌羟脯氨酸( hydroxypro-line, Hydro)的含量；HE和Massson染色观察心肌纤维化的程度,并计算心肌胶原容积分数( collagen volume fraction, CVF)；运用RT-PCR 方法测定I型胶原( CollagenI) mRNA表达。结果模型组与溶媒对照组比较, CWI 和心肌中Hydro 的含量明显增加( P<0.01),心肌细胞间可见较多的纤维组织,相应地,CVF和左心室CollagenImRNA表达明显增加( P<0.01)。结论小鼠皮下注射异丙肾上腺素成功制备了心肌纤维化模型,该法简单、经济、可靠。
목적피하주사이병신상선소제비소서심기섬유화모형병대기진행평개。방법장곤명충소서수궤분위심기섬유화모형조화용매대조조,매조10지。모형조소서피하주사5 mg·kg-1이병신상선소1 d후,이2.5 mg·kg-1유지지30 d。용매대조조동법피하주사등량적생리염수。30 d후경추탈구처사소서,취심장칭중,계산심중지수( cardiac weight index, CWI)；소화법측정심기간포안산( hydroxypro-line, Hydro)적함량；HE화Massson염색관찰심기섬유화적정도,병계산심기효원용적분수( collagen volume fraction, CVF)；운용RT-PCR 방법측정I형효원( CollagenI) mRNA표체。결과모형조여용매대조조비교, CWI 화심기중Hydro 적함량명현증가( P<0.01),심기세포간가견교다적섬유조직,상응지,CVF화좌심실CollagenImRNA표체명현증가( P<0.01)。결론소서피하주사이병신상선소성공제비료심기섬유화모형,해법간단、경제、가고。
Aim To establish and evaluate a mouse model of my-ocardial fibrosis by hypodermic injection of isoprenaline. Meth-ods Kunming mice were randomly divided into 2 groups, namely, the myocardial fibrosis model group and the control group, 10 mice in each group. The mice in the model group were given isoprenaline 5 mg · kg-1 by hypodermic injection. From the following day, the dose of isoprenaline was reduced to 2. 5 mg·kg-1 , and lasted for 30 days. The mice in the control group were treated with the physiological saline in the same way. Final-ly, heart weight was then weighed and the cardiac weight index (CWI) was calculated. Hydroxyproline (Hydro) level in myo-cardium was determined by a colorimetric method. HE and Mas-son’s trichrome staining were used to estimate the extent of myo-cardial fibrosis and calculate the collagen volume fraction ( CVF) . RT-PCR was used to measure the myocardial Collagen I mRNA expression. Results Compared with the control group, the CWI and Hydro content in the myocardial tissues in the model group were increased(P<0.01). The content of col-lagen in the myocardial tissues and the CVF were increased obvi-ously(P<0.01). The RT-PCR results showed that the left ven-tricle Collagen I mRNA expression in the model group increased obviously( P<0.01 ) . Conclusion Isoprenaline-induced myo-cardial fibrosis model has been established and the method is very simple, economic and reliable.